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標題: Secoaggregatalactone-A from Lindera aggregata induces apoptosis in human hepatoma hep G2 cells
作者: Lin, C.T.
Chu, F.H.
Chang, S.T.
Chueh, P.J.
Su, Y.C.
Wu, K.T.
Wang, S.Y.
關鍵字: secoaggregatalactone A;apoptosis;cytotoxicity;Lindera aggregata;Lauraceae;mitochondrial pathway;cytochrome-c release;water extract;stem wood;strychnifolia;root
Project: Planta Medica
期刊/報告no:: Planta Medica, Volume 73, Issue 15, Page(s) 1548-1553.
A new secobutanolide, secoaggregatalactone A (1) was isolated from the leaves of Lindera aggregata. Results obtained from the cytotoxicity assay revealed that secoaggregatalactone A exhibited a noticeable cytotoxicity (EC50 = 6.61 mu g/mL; 22.1 mu M) against the human hepatoma cell line (Hep G2 cell line). According to morphological observations, flow cytometric analysis, and DNA fragmentation analysis, it was proven that the cytotoxicity of secoaggregatalactone A on human cells was due to apoptosis. Moreover, based on the results from the protein expression assay and confocal laser scanning microscope observations, it is assumed that secoaggregatalactone A induced apoptosis through the mitochondria pathway by way of cleavage of Bit to release cytochrome C and activate caspases-9 and -3, and then degradation of PARP.
ISSN: 0032-0943
DOI: 10.1055/s-2007-993739
Appears in Collections:生物醫學研究所

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