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標題: Differential expression of U2AF(35) in the arthritic joint of avian reovirus-infected chicks
作者: Fan, Y.H.
Lin, C.D.
Chiou, S.H.
Chow, K.C.
Chi, Y.S.
Lee, L.H.
Shien, J.H.
Shieh, H.K.
關鍵字: avian reovirus (ARV);bone morphogenetic protein-2 (BMP-2);matrix;metalloproteinase-2 (MMP-2);small subunit of U2 snRNP;auxiliary factor;(U2AF(35) or U2AF1);viral arthritis (VA);bone morphogenetic protein-2;messenger-rna expression;splicing factor;u2af(35);rheumatoid-arthritis;viral-arthritis;virus;gene;tenosynovitis;localization;collagenase
Project: Veterinary Immunology and Immunopathology
期刊/報告no:: Veterinary Immunology and Immunopathology, Volume 114, Issue 1-2, Page(s) 49-60.
To identify cell types and genes that are differentially expressed during immunopathogenesis of avian reovirus (ARV)-induced viral arthritis (VA), we inoculated arthrotropic strain SI 133 of ARV into 1-day-old broilers, and examined tissue histology as well as RNA expression at different days post-inoculation (PI). Using immunohistochemical staining, we detected many CD68 expressing macrophages in and around the blood vessels of the arthritic joints. By RT-PCR, we found that expression of matrix metalloproteinase-2 (MMP-2) and bone morphogenetic protein-2 (BMP-2) was induced earlier in footpads and hock joints of ARV-infected chickens. By employing suppression subtractive hybridization (SSH) technique and RT-PCR, we further identified that small subunit of U2 snRNP auxiliary factor (U2AF(35) or U2AF1) mRNA was differentially induced in the joint of ARV-infected chickens. By in situ hybridization (ISH), mRNA signals of U2AF(35) and BMP-2 were located in chondrocytes within/near the epiphyseal plate and secondary center of ossification, and in epidermal cells and dermal fibroblast-like cells of arthritic joints. In addition, U2AF(35) mRNA was expressed in the inflammatory infiltrates of the bone marrow of ARV-infected arthritic joints, while MMP-2 was mainly detected in chondrocytes. Interestingly, among U2AF(35), MMP-2, and BMP-2 that were differentially expressed in the joint of ARV-infected chickens, only U2AF(35) induction correlated well with arthritic manifestation. Because U2AF(35) may assist in mRNA splicing of proinflammatory chemokines and cytokines, our results indicated that U2AF(35) induction might play an immunopathological role in ARV-induced arthritis. This study has first associated U2AF(35) to viral arthritis. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 0165-2427
DOI: 10.1016/j.vetimm.2006.07.003
Appears in Collections:生物醫學研究所

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