Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40502
標題: Prodigiosin-induced cytotoxicity involves RAD51 down-regulation through the JNK and p38 MAPK pathways in human breast carcinoma cell lines
作者: Lu, Chien-Hsing
Lin, Shin-Chang
Yang, Shu-Yi
Pan, Mu-Yun
Lin, Yun-Wei
Hsu, Chun-Yi
Wei, Yu-Hong
Chang, Jo-Shu
Chang, Chia-Che
關鍵字: RAD51;Prodigiosin;DNA double-strand breaks;Homologous recombination repair pathway;Breast cancer
Project: Toxicology Letters, Volume 212, page(s) 83– 89.
摘要: 
RAD51 is essential for homologous recombination (HR)-mediated repair of DNA double-strand breaks
(DSBs) in mammalian cells. RAD51 is an attractive target for anticancer drugs, given high RAD51 levels are
frequently observed in many human tumors and associated with increased resistance to DSBs-inducing
chemotherapeutics. Prodigiosin is a bacterial tripyrrole pigment with potent anticancer activity and also
provokes DSBs. We hereby aimed to elucidate the role of RAD51 in prodigiosin-induced cytotoxicity.
Prodigiosin was found to down-regulate RAD51 in multiple human breast carcinoma cell lines irrespective
of p53 status. Mechanistically, prodigiosin lowered RAD51 mRNA expression, whereas blockade of
proteasome-mediated degradation failed to restore RAD51 levels following prodigiosin treatment. In
addition, prodigiosin triggered phosphorylation of JNK and p38 MAPK, while pharmacological inhibition
of JNK or p38 MAPK attenuated prodigiosin-mediated inhibition of RAD51 mRNA expression. Lastly,
cells with enforced RAD51 expression showed increased resistance to prodigiosin-induced cytotoxicity
as well as inhibition of colony formation. Collectively, we conclude that RAD51 down-regulation represents
one of the modes of prodigiosin’s cytotoxic action, ostensibly by augmenting the genotoxic effect
of prodigiosin through suppression of RAD51-mediated HR repair. Our findings further implicate the
use of prodigiosin to potentiate the cytotoxicity of DSB-inducing chemotherapeutics through RAD51
down-regulation.
URI: http://hdl.handle.net/11455/40502
ISSN: 0378-4274
DOI: 10.1016/j.toxlet.2012.05.002
Appears in Collections:生物醫學研究所

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