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標題: Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography
作者: Liau, Bing-Chung
Hong, Siang-En
Chang, Li-Pin
Shen, Chun-Tin
Li, Yu-Chun
Wu, Ying-Ping
Jong, Ting-Ting
Shieh, Chwen-Jen
Hsu, Shih-Lan
Chieh-Ming, J.Chang
關鍵字: Nannochloropsis oculata;Zeaxanthin;Supercritical fluid extraction;Elution chromatography;Anti-oxidative capacities
出版社: Elsevier B.V.
Project: Separation and Purification Technology, Volume 78, Issue 1, Page(s) 1-8.
Microalgae is a potent source of carotenoids and is regarded as functional foods consumed by people worldwide. In this study, supercritical fluid extraction coupled with elution chromatography was employed to recover six carotenoids including zeaxanthin from Nannochloropsis oculata and extraction efficiency of zeaxanthin was determined. The amount of the zeaxanthin (400 mg/g) in the elution fraction increased more than twenty folds from that of the SFE extract (13.17 mg/g). The extracts were subsequently subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay, 2-2'-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid (ABTS)) assay and adult retinal pigment epithelium-19 (ARPE-19) cells. Experimental results demonstrate that other components than zeaxanthin may play a crucial role in the anti-oxidative ability of the extracts from N. oculata. This work provides a useful method to recover bioactive anti-oxidative zeaxanthin from microalgae of N. oculata by using modified supercritical fluids extraction.
ISSN: 1383-5866
DOI: 10.1016/j.seppur.2011.01.008
Appears in Collections:化學工程學系所

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