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標題: Supercritical carbon dioxide anti-solvent precipitation of anti-oxidative zeaxanthin highly recovered by elution chromatography from Nannochloropsis oculata
作者: Cho, Yueh-Cheng
Cheng, Jia-Hui
Hsu, Shih-Lan
Hong, Siang-En
Lee, Tse-Min
Chieh-Ming, J.Chang
關鍵字: Nannochloropsis oculata;Zeaxanthin;Column fractionation;Supercritical anti-solvent precipitation
出版社: Elsevier B.V.
Project: Separation and Purification Technology, Volume 78, Issue 3, Page(s) 274-280.
Zeaxanthin, a kind of xanthophylls, rich in Marigold flowers and a few micro algae, acts as a strong antioxidant capable of protecting human eye-sight from age-related macular degeneration. This study examined elution chromatography coupled with supercritical anti-solvent precipitation applied for generation of zeaxanthin-rich particulates from micro-algal Nannochloropsis oculata species. Crude of the ultrasonic acetone extract subjected to the column fractionation successfully yields a fraction containing 303.02 mg/g (30%) of zeaxanthin with recovery of 90%. Supercritical anti-solvent precipitation of the algal solution at 200 bar and 313 K was used in producing submicron-sized particulates of 50% zeaxanthin. These extracts were subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate free-radical assay and human adult retinal pigment epithelium-19 cells. This work provided a fast and environmental benign process to generate anti-oxidative capable algal micronized powders from the N. oculata.
ISSN: 1383-5866
DOI: 10.1016/j.seppur.2011.02.017
Appears in Collections:化學工程學系所

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