Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/40995
DC FieldValueLanguage
dc.contributor.authorWu, Tsung-Taen_US
dc.contributor.authorLin, Sung-Chyren_US
dc.contributor.authorShaw, Jei-Fuen_US
dc.contributor.other國立中興大學化學工程學系zh_TW
dc.contributor.otherNational Chung Hsing University,Department of Chemical Engineeringen_US
dc.date2011-7zh_TW
dc.date.accessioned2014-06-06T08:04:47Z-
dc.date.available2014-06-06T08:04:47Z-
dc.identifier.issn1359-5113zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/40995-
dc.description.abstractIn this study an integrated process for the concomitant purification and immobilization of recombinant Picrophilus torridus trehalose synthase for the synthesis of trehalose was reported. A highly porous crosslinked polystyrene divinylbezene-based metal chelator was used for the purification and immobilization of the poly(His)-tagged enzyme. While the Cu(II)-loaded adsorbent showed the highest protein adsorption capacity, 5.39 0.03 mg protein/g, the Co(II)-loaded adsorbent exhibited the highest specificity for the adsorption of the poly(His)-tagged trehalose synthase, giving a specific activity of 3.98 0.50 U/mg protein, more than fourfold higher than that of the Cu(II)-loaded adsorbent. No significant differences in optimal operation pH, temperature, and thermostability were observed upon immobilization. Under optimal reaction conditions, a trehalose conversion yield of 64.1% was obtained within 6 h. A residual activity of approximately 80% was maintained after 24 cycles in a repeated-batch operation. The decline in residual activity was primarily due to the desorption of the recombinant trehalose synthase. The proposed integrated approach for enzyme purification and immobilization with the metal chelator is promising for the development of industrial processes for trehalose production.en_US
dc.language.isoen_USzh_TW
dc.publisherElsevier Ltd.en_US
dc.relationProcess Biochemistry, Volume 46, Issue 7, Page(s) 1481-1485.en_US
dc.subjectImmobilized enzymeen_US
dc.subjectTrehaloseen_US
dc.subjectTrehalose synthaseen_US
dc.titleIntegrated process for the purification and immobilization of recombinant trehalose synthase for trehalose productionen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.procbio.2011.03.023zh_TW
dc.contributor.catalogerMiao-zhen Luoen_US
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
Appears in Collections:化學工程學系所
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