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標題: A continuous ultrasound-assisted packed-bed bioreactor for the lipase-catalyzed synthesis of caffeic acid phenethyl ester
作者: Chen, H.C.
Kuo, C.H.
Twu, Y.K.
Chen, J.H.
Chang, C.M.J.
Liu, Y.C.
Shieh, C.J.
關鍵字: lipase;packed-bed reactor;phenolic acid;response surface methodology;ultrasonication;enzymatic-synthesis;reactor;oil;hydrolysis;kinetics;solvent;energy;cotton
Project: Journal of Chemical Technology and Biotechnology
期刊/報告no:: Journal of Chemical Technology and Biotechnology, Volume 86, Issue 10, Page(s) 1289-1294.
BACKGROUND: The focus of this paper is the ultrasound-assisted synthesis of caffeic acid phenethyl ester (CAPE) from caffeic acid and phenyl ethanol in a continuous packed-bed bioreactor. Immobilized Novozym (R) 435 (from Candida antarctica) is used as the catalyst. A three-level-three-factor Box-Behnken design and a response surface methodology (RSM) are employed to evaluate the effects of temperature, flow rate, and ultrasonic power on the percentage molar conversion of CAPE. RESULTS: Based on ridge max analysis, it is concluded that the optimum condition for synthesis is reaction temperature 72.66 degrees C, flow rate 0.046 mL min(-1), and ultrasonic power 1.64 W cm(-2). The expected molar conversion value is 97.84%. An experiment performed under these optimal conditions resulted in a molar conversion of 92.11 +/- 0.75%. The enzyme in the bioreactor was found to be stable for at least 6 days. CONCLUSIONS: The lipase-catalyzed synthesis of CAPE by an ultrasound-assisted packed-bed bioreactor uses mild reaction conditions. Enzymatic synthesis of CAPE is suitable for use in the nutraceutical and food production industries. (C) 2011 Society of Chemical Industry
ISSN: 0268-2575
DOI: 10.1002/jctb.2661
Appears in Collections:化學工程學系所

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