Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41140
DC FieldValueLanguage
dc.contributor.authorLi, S.Y.en_US
dc.contributor.author林松池zh_TW
dc.contributor.authorChang, B.Y.en_US
dc.contributor.authorLin, S.C.en_US
dc.date2006zh_TW
dc.date.accessioned2014-06-06T08:05:00Z-
dc.date.available2014-06-06T08:05:00Z-
dc.identifier.issn0168-1656zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41140-
dc.description.abstractTwin-arginine translocation (Tat) pathway is capable of secreting fully folded proteins into the periplasm, of Gram-negative bacteria and may thus be an ideal system for the expression of active cofactor-containing proteins. However, the applications of Tat system for such purpose have been plagued by low translocation efficiencies. In this study, we demonstrate that the coexpression of a soluble chaperone, TorD, in conjunction with the TorA signal peptide, the translocation efficiency of GFP can be enhanced by more than three-fold. The enhancement in translocation efficiency is believed to be a result of reduced proteolysis mediated by the binding of TorD toward the TorA signal peptide. We believe this approach can be further exploited for the expression and secretion of other heterologous proteins as well as traditional Tat substrate proteins. (c) 2005 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Biotechnologyen_US
dc.relation.ispartofseriesJournal of Biotechnology, Volume 122, Issue 4, Page(s) 412-421.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.jbiotec.2005.09.011en_US
dc.subjecttwin-arginine pathwayen_US
dc.subjectgreen fluorescence proteinen_US
dc.subjectsecretionen_US
dc.subjectTorDen_US
dc.subjectgreen fluorescent proteinen_US
dc.subjectarginine translocation pathwayen_US
dc.subjecthigh-levelen_US
dc.subjectexpressionen_US
dc.subjectescherichia-colien_US
dc.subjectsignal peptideen_US
dc.subjectexport pathwayen_US
dc.subjectcomplexen_US
dc.subjectsystemen_US
dc.subjectbacteriaen_US
dc.subjectidentificationen_US
dc.titleCoexpression of TorD enhances the trans-port of GFP via the TAT pathwayen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jbiotec.2005.09.011zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:化學工程學系所
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