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標題: General approach for the development of high-performance liquid chromatography methods for biosurfactant analysis and purification
作者: Lin, S.C.
Chen, Y.C.
Lin, Y.M.
關鍵字: ultrafiltration;surfactants;lipopeptides;surfactin;bacillus-licheniformis jf-2;surface-active compounds;microbial;surfactants;structural-analysis;subtilis;ultrafiltration;microorganisms;hydrocarbons;degradation;recovery
Project: Journal of Chromatography A
期刊/報告no:: Journal of Chromatography A, Volume 825, Issue 2, Page(s) 149-159.
A general approach for the development of HPLC methods for biosurfactant analysis and purification was proposed. By comparing the chromatograms of the cell-free fermentation broth, the ultrafiltration filtrate, and the ultrafiltration filtrate of a methanal-surfactant mixture, the peaks corresponding to biosurfactants can be identified without any prior structural information of the biosurfactants. It can be assumed that the peaks observed only on the chromatogram of the filtrate of methanol-surfactant mixture but not on the chromatogram of the filtrate are biosurfactant peaks. This approach can be applied for the development of a HPLC assay for any biosurfactants as long as the concentration of biosurfactants in the fermentation broth is higher than the critical micelle concentration. The HPLC methods thus developed can also be adapted for the preparation of homogeneous biosurfactant samples useful for chemical analysis for the elucidation of chemical structure of biosurfactants and for the determination of the physical properties of biosurfactants. (C) 1998 Elsevier Science B.V. All rights reserved.
ISSN: 0021-9673
DOI: 10.1016/s0021-9673(98)00709-2
Appears in Collections:化學工程學系所

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