Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41167
標題: Production of N-carbamoyl-D-hydroxyphenylglycine by D-hydantoinase activity of a recombinant Escherichia coli
作者: Chen, Y.C.
林松池
Yin, B.D.
Lin, S.C.
Hsu, W.H.
關鍵字: calcium alginate;D-hydantoinase;D-p-hydroxyphenylglycine;Escherichia;coli;immobilization;d-p-hydroxyphenylglycine;d-amino acids;microbial transformation;pseudomonas-putida;calcium alginate;agrobacterium sp;microorganisms;enzyme;expression;gene
Project: Process Biochemistry
期刊/報告no:: Process Biochemistry, Volume 35, Issue 3-4, Page(s) 285-290.
摘要: 
Recombinant Escherichia coli BL21 (DE3) harbouring plasmid pET36 encoding D-hydantoinase from Pseudomonas putida were used as biocatalyst for the production of N-carbamoyl-D-hydroxyphenylglycine from DL-p-hydroxyphenylhydantoin. The optimum D-hydantoinase activity was observed at 40 degrees C and pH 8.5. At a substrate concentration of 300 mg/l, an initial reaction rate of 24.52 mM/h g cells was obtained and 91% of the substrate was converted into product after a 24-h reaction. Recombinant cells were immobilized within calcium alginate beads with diameters ranging from 2 to 3 mm. The specific activity of the immobilized cells increased with cell loads, probably due to reduced mass transfer resistance. The immobilized cells also exhibited an optimal pH of 8.5. However, under the conditions described above, the initial reaction rate with the immobilized cells as the biocatalysts was reduced by 87% to 3.19 mM/h g cells, probably due to the formation of cell aggregates inaccessible to substrate. Thermostability and reusability of D-hydantoinase were increased upon immobilization. The initial reaction rate with immobilized cells was increased with temperature at least up to 60 degrees C. More than 95% of the D-hydantoinase activity was recovered after three cycles for the immobilized cells, compared to 22% for the free cell systems. (C) 1999 Elsevier Science Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/41167
ISSN: 0032-9592
DOI: 10.1016/s0032-9592(99)00066-7
Appears in Collections:化學工程學系所

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