Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41261
DC FieldValueLanguage
dc.contributor.authorSuen, S.Y.en_US
dc.contributor.author孫幸宜zh_TW
dc.contributor.authorLin, S.Y.en_US
dc.contributor.authorChiu, H.C.en_US
dc.date2000zh_TW
dc.date.accessioned2014-06-06T08:05:14Z-
dc.date.available2014-06-06T08:05:14Z-
dc.identifier.issn0888-5885zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41261-
dc.description.abstractBy using different spacer arms to immobilize Cibacron Blue 3GA onto regenerated cellulose membrane discs, this work investigated their effects on ligand density and corresponding lysozyme adsorption. When EGDGE alone was adopted as a spacer arm, using 0.1 M EGDGE without the formation of liquid film in its reaction with the membrane disc led to higher adsorption capacities. When EGDGE was combined with another reagent with two end functional groups as a spacer arm, EGDGE + 1,4-diaminobutane performed better in both ligand density and adsorption capacity. In addition, the ligand densities using the combinations of EGDGE with another reagent were generally lower than those from EGDGE alone, whereas the ligand utilization percentages were higher. Moreover, adding a spacer arm did not affect the binding strength between lysozyme and Cibacron Blue 3GA, regardless of the type of spacer arms and their length, and the reaction condition.en_US
dc.language.isoen_USzh_TW
dc.relationIndustrial & Engineering Chemistry Researchen_US
dc.relation.ispartofseriesIndustrial & Engineering Chemistry Research, Volume 39, Issue 2, Page(s) 478-487.en_US
dc.relation.urihttp://dx.doi.org/10.1021/ie990421ten_US
dc.subjecthollow-fiber membranesen_US
dc.subjectaffinity membranesen_US
dc.subjectimmunoaffinityen_US
dc.subjectchromatographyen_US
dc.subjectprotein chromatographyen_US
dc.subjectimmunoglobulin-gen_US
dc.subjectpurificationen_US
dc.subjectbreakthroughen_US
dc.subjectseparationen_US
dc.titleEffects of spacer arms on Cibacron Blue 3GA immobilization and lysozyme adsorption using regenerated cellulose membrane discsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1021/ie990421tzh_TW
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
Appears in Collections:化學工程學系所
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