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標題: Isolation of lysozyme from hen egg albumen using glass fiber-based cation-exchange membranes
作者: Chiu, H.C.
Lin, C.W.
Suen, S.Y.
關鍵字: glass fiber membrane;monophenyl trimethoxysilane;sulphonation;cation-exchange membrane;inorganic hybrid materials;ion-exchange;protein separation;charged;membrane;plasmid dna;chromatography;purification;adsorbers;module;white
Project: Journal of Membrane Science
期刊/報告no:: Journal of Membrane Science, Volume 290, Issue 1-2, Page(s) 259-266.
In this study, porous glass fiber membranes were coated with monophenyl trimethoxysilane (MPh) and then sulphonated by chlorosulphonic acid to prepare the cation-exchange membranes with sulphonic acid groups. Different MPh-coating times were tested and the properties of the resulting membranes such as contact angle, FTIR spectrum, conductivity, and ion-exchange capacity were measured. It was found that the optimal MPh-coating time was 60 min and the related ion-exchange capacity was 49.5 mu equiv./disc. The modified membrane under the optimal MPh-coating condition was adopted for lysozyme isolation. The results were compared with those for the commercial cation-exchange membrane with sulphonic acid groups (ICE 450 unsupported membrane). Although the prepared membrane exhibited less adsorption capacity than ICE 450 unsupported membrane in the batch lysozyme adsorption experiment, it showed lower non-specific binding ratio under higher salt concentration. In the flow process isolating lysozyme from hen egg albumen, the purification effectiveness obtained using the prepared cation-exchange membrane was superior to the ICE 450 unsupported membrane. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 0376-7388
DOI: 10.1016/j.memsci.2006.12.042
Appears in Collections:化學工程學系所

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