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標題: Improved retention of endothelial cells seeded on polyurethane small-diameter vascular grafts modified by a recombinant RGD-containing protein
作者: Hsu, S.H.
Sun, S.H.
Chen, D.C.H.
關鍵字: polyurethane;small-diameter vascular grafts;RGD-containing proteins;endothelial cell seeding;cell retention;genetic engineering;peptides;adhesion;culture;attachment;prostheses;surface
Project: Artificial Organs
期刊/報告no:: Artificial Organs, Volume 27, Issue 12, Page(s) 1068-1078.
Sponge-type small-diameter vascular grafts were fabricated from a medical-grade polyurethane, Pellethane 2363-80A, by utilization of a salt casting technique. The grafts were compliance matched with a storage modulus of 0.53 +/- 0.08 MPa. The luminal surface of grafts was modified with a thin layer (similar to40 mum) of gelatin crosslinked by epoxide. Then a special Arg-Gly-Asp (RGD)-containing recombinant protein, named CBD-RGD (cellulose binding domain RGD-containing protein), was coated onto the gelatin layer. The platelet adhesion and activation on such a gelatin/CBD-RGD modified surface was significantly reduced. Human umbilical vein endothelial cells were seeded more efficiently onto the modified grafts. There was also a substantial reduction in the subsequent loss of cells from the graft surface following perfusion in vitro. The cell number retained on the modified graft was enhanced by three times after 1 h of perfusion, and by eight times after 3 h of perfusion (retention rate similar to63%). The retention after 3 h of perfusion could be further increased to nearly 100% if the lined endothelium on gelatin/CBD-RGD modified graft was cultured for another week before perfusion. The modified surface was also shown to help canine external jugular vein endothelial cells to maintain the round cell morphology in vitro.
ISSN: 0160-564X
DOI: 10.1111/j.1525-1594.2003.07141.x
Appears in Collections:化學工程學系所

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