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標題: The effect of an RGD-containing fusion protein CBD-RGD in promoting cellular adhesion
作者: Hsu, S.H.
Chu, W.P.
Lin, Y.S.
Chiang, Y.L.
Chen, D.C.H.
Tsai, C.L.
關鍵字: RGD-containing peptides;polyurethane;poly(L-lactide);poly(lactide-co-glycolide);extracellular-matrix proteins;endothelial-cells;peptides;fibronectin;attachment;receptor;culture;chondrocytes;cartilage;surface
Project: Journal of Biotechnology
期刊/報告no:: Journal of Biotechnology, Volume 111, Issue 2, Page(s) 143-154.
The effect of a recombinant RGD (arginine-glycine-aspartic acid)-containing fusion protein, cellulose-binding domain (CBD)RGD, on the cellular adhesion to a biomedical polyurethane (PU) was evaluated. A series of different cell lines, as well as freshly harvested animal cells, were grown on the PU surfaces with or without CBD-RGD, in serum or serum-free media. The results showed that the enhancement of cellular attachment by CBD-RGD varied with cell types. This is believed to be a result of the unique integrin receptors on each type of cell surface. The existence of certain divalent ions (Mg2+ and Mn2+) may increase the efficacy of the CBD-RGD, in a cell type-dependent manner. The fusion protein was also found to inhibit the platelet activation. The effect of CBD-RGD was further examined in two other substrate materials, poly(L-lactide) (PLLA) and poly(lactide-co-glycolide) (PLGA). The effect on cellular adhesion correlated with the amount of CBD-RGD physically adsorbed on the material surface. (C) 2004 Elsevier B.V. All rights reserved.
ISSN: 0168-1656
DOI: 10.1016/j.jbiotec.2004.03.014
Appears in Collections:化學工程學系所

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