Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41469
DC FieldValueLanguage
dc.contributor.authorChang, H.C.en_US
dc.contributor.author張厚謙zh_TW
dc.contributor.authorLin, Y.C.en_US
dc.contributor.authorKuo, C.T.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-06T08:05:33Z-
dc.date.available2014-06-06T08:05:33Z-
dc.identifier.issn1369-703Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41469-
dc.description.abstractTransport of biomolecules within cells plays a critical role in the maintenance of the cellular functions. Compared to diffusion in aqueous phase, cytoplasmic diffusion is partly retarded by the elevated viscosity of cytosol. In addition, intracellular diffusion is affected by cytoplasmic physical properties such as tortuosity, binding, and molecule crowding. This work develops a two dimensional mathematical model describing the intracellular diffusion and yielding probe concentration distribution within the cell in time course. Initially, cytoplasmic mobility is quantified as apparent diffusion coefficient (ADC), with which all the diffusion resistances encountered are accounted for. Binding, however, as an important diffusion resistance, is then isolated from all others included in ADC. Using the theoretical analysis from the literature, furthermore we quantify the contribution of steric hindrance in retarded cytoplasmic mobility. In all, three major diffusion resistances including molecular binding to cytoarchitecture, molecule detouring caused by cytoskeleton obstacles, and elevated cytosol viscosity are elucidated and quantified in this work. All of these factors play significant roles in mobility retardness in intracellular transport. (C) 2008 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical Engineering Journalen_US
dc.relation.ispartofseriesBiochemical Engineering Journal, Volume 41, Issue 3, Page(s) 217-227.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.bej.2008.04.021en_US
dc.subjectdiffusionen_US
dc.subjectmodellingen_US
dc.subjectmass transferen_US
dc.subjectviscosityen_US
dc.subjectcytoplasmic bindingen_US
dc.subjectcytoskeleton hindranceen_US
dc.subjecttranslational diffusionen_US
dc.subjectphysical-propertiesen_US
dc.subjectcell cytoplasmen_US
dc.subjectpermeabilityen_US
dc.subjectmobilityen_US
dc.subjectnucleusen_US
dc.subjectsizeen_US
dc.subjectpolarizationen_US
dc.subjectmovementen_US
dc.subjectsoluteen_US
dc.titleA two-dimensional diffusion model quantifying intracellular transport with independent factors accounting for cytosol viscosity, binding, and steric hindranceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bej.2008.04.021zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:化學工程學系所
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