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標題: Pressurized liquid extraction of bioactive compounds from Taiwan pines
作者: Lin, C.C.
Chang, C.J.
Lin, S.C.
關鍵字: pine-needles;pressurized liquid extraction;Taguchi method;response;surface methodology;proanthocyanidins;antioxidative activity;solvents
Project: Journal of the Chinese Institute of Chemical Engineers
期刊/報告no:: Journal of the Chinese Institute of Chemical Engineers, Volume 35, Issue 4, Page(s) 399-407.
An experiment combining the Taguchi methodology with the response surface methodology to optimize the Pressurized Liquid Extraction (PLE) of proanthocyanidins from Taiwan pines was conducted. A L-18 orthogonal array was selected to investigate the total yield and the amount of proanthocyanidins. Experimental results showed that the extraction temperature most significantly affected the efficiency of extraction, but that a higher temperature also caused decomposition of bioactive components. Optimum conditions were identified as 20% EtOH, 140degreesC, 750 psig, and 1.5 h. The PLE total yield, proanthocyanidins content and total flavonoids were 2.09, 2.07, and 2.01 times greater than those obtained using a 50% EtOH conventional extraction method. Hot pressurized extraction has the advantages of low energy and high yield of bioactive compounds. The antioxidant activities of several extracts from two Taiwan pine-needles were individually evaluated for scavenging of DPPH free radicals and superoxide anion radicals, and for the inhibition of lipid peroxidation. The results revealed that both PLE M (Pinus morrisonicola) and PLE T (Pinus taiwanensis) extracted from two fresh pine needles scavenged 78.91% of DPPH free radicals. Air-dried pine needles also strongly inhibited superoxide anion radicals. The purification of four anthocyanidins from a PLE T extract was initially tested using HP-20 resin solid phase extraction and showed little enhancement. The isolation, purification and antioxidative mechanism of active components from pine needles need to be further investigated.
ISSN: 0368-1653
Appears in Collections:化學工程學系所

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