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|標題:||Pressurized liquid extraction of bioactive compounds from Taiwan pines||作者:||Lin, C.C.
|關鍵字:||pine-needles;pressurized liquid extraction;Taguchi method;response;surface methodology;proanthocyanidins;antioxidative activity;solvents||Project:||Journal of the Chinese Institute of Chemical Engineers||期刊/報告no：:||Journal of the Chinese Institute of Chemical Engineers, Volume 35, Issue 4, Page(s) 399-407.||摘要:||
An experiment combining the Taguchi methodology with the response surface methodology to optimize the Pressurized Liquid Extraction (PLE) of proanthocyanidins from Taiwan pines was conducted. A L-18 orthogonal array was selected to investigate the total yield and the amount of proanthocyanidins. Experimental results showed that the extraction temperature most significantly affected the efficiency of extraction, but that a higher temperature also caused decomposition of bioactive components. Optimum conditions were identified as 20% EtOH, 140degreesC, 750 psig, and 1.5 h. The PLE total yield, proanthocyanidins content and total flavonoids were 2.09, 2.07, and 2.01 times greater than those obtained using a 50% EtOH conventional extraction method. Hot pressurized extraction has the advantages of low energy and high yield of bioactive compounds. The antioxidant activities of several extracts from two Taiwan pine-needles were individually evaluated for scavenging of DPPH free radicals and superoxide anion radicals, and for the inhibition of lipid peroxidation. The results revealed that both PLE M (Pinus morrisonicola) and PLE T (Pinus taiwanensis) extracted from two fresh pine needles scavenged 78.91% of DPPH free radicals. Air-dried pine needles also strongly inhibited superoxide anion radicals. The purification of four anthocyanidins from a PLE T extract was initially tested using HP-20 resin solid phase extraction and showed little enhancement. The isolation, purification and antioxidative mechanism of active components from pine needles need to be further investigated.
|Appears in Collections:||化學工程學系所|
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