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標題: Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX
作者: Wang, S.J.
Lin, H.M.
Wang, X.R.
Hsiung, K.P.
Liu, Y.C.
關鍵字: reactive dyes;immunochromatographic test;optimal reaction conditions;response surface methodology;immunochromatographic assay;schistosomiasis diagnosis;urinary albumin;rapid detection;whole-blood;strip test;immunoassay;system;chromatography;mansoni
Project: Analytical Biochemistry
期刊/報告no:: Analytical Biochemistry, Volume 361, Issue 2, Page(s) 190-196.
For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 (mol dye/mol antibody), and reaction time 45.6 min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity. (c) 2006 Elsevier Inc. All rights reserved.
ISSN: 0003-2697
DOI: 10.1016/j.ab.2006.11.026
Appears in Collections:化學工程學系所

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