Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX

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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41671

標題:	Optimized dyeing conditions of immunoprotein with reactive dye Procion Blue MX-7RX
作者:	Wang, S.J. 劉永銓 Lin, H.M. Wang, X.R. Hsiung, K.P. Liu, Y.C.
關鍵字:	reactive dyes;immunochromatographic test;optimal reaction conditions;response surface methodology;immunochromatographic assay;schistosomiasis diagnosis;urinary albumin;rapid detection;whole-blood;strip test;immunoassay;system;chromatography;mansoni
Project:	Analytical Biochemistry
期刊/報告no：:	Analytical Biochemistry, Volume 361, Issue 2, Page(s) 190-196.
摘要:	For investigating the feasibility of using reactive dyes as an immunoassay marker, a dichlorine triazine dye, Procion Blue MX-7RX, was employed to stain the antibody against human serum albumin (anti-HSA). With the color intensity revealed in the immunochromatographic test strip as the objective variables, the optimal dyeing conditions were found as follows: pH 11.4, temperature 35.7 degrees C, molar ratio 188 (mol dye/mol antibody), and reaction time 45.6 min. The dyed-anti-HSA revealed a maximal color intensity of 8738 without apparent loss of antigen binding affinity. (c) 2006 Elsevier Inc. All rights reserved.
URI:	http://hdl.handle.net/11455/41671
ISSN:	0003-2697
DOI:	10.1016/j.ab.2006.11.026
Appears in Collections:	化學工程學系所

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