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|標題:||Effect of oxygen enrichment aeration on penicillin G acylase production in high cell density culture of recombinant E-coli||作者:||Liu, Y.C.
|關鍵字:||fed-batch system;escherichia-coli;dissolved-oxygen;cultivation;plasmids;growth;gene||Project:||Bioprocess Engineering||期刊/報告no：:||Bioprocess Engineering, Volume 21, Issue 3, Page(s) 227-230.||摘要:||
A strain of E. coli 9633 harboring pGL-5 is employed to develop the process producing penicillin G acylase (PGA). Medium studies show that 0.8% glucose is a proper initial carbon source concentration in the start medium. Whereas, study of nitrogen sources in the production medium exhibits that 2% soybean meat hydrolysate and 0.5% casein hydrolysate give the best result in terms of cell concentration. High cell density culture is carried out through the application of oxygen enrichment aeration (OEA). With the treatment of OEA, the cell concentration and specific PGA activity raise to 2.1 and 1.4 times, respectively, as compared to those without OEA. In a 751 fermenter, the cell concentration can reach 323 (g wet cell weight/l) with a specific PGA activity of 47 (IU/g w.w.) after 24 h under OEA treatment. This means that the PGA activity of the broth can reach 15 (IU/ml).
|Appears in Collections:||化學工程學系所|
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