Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41718
DC FieldValueLanguage
dc.contributor.authorTsai, Y.C.en_US
dc.contributor.author蔡毓楨zh_TW
dc.contributor.authorHuang, J.D.en_US
dc.contributor.authorChiu, C.C.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:05:48Z-
dc.date.available2014-06-06T08:05:48Z-
dc.identifier.issn0956-5663zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41718-
dc.description.abstractA novel amperometric ethanol biosensor was constructed using alcohol dehydrogenase (ADH) physically immobilized within poly(vinyl alcohol)-multiwalled carbon nanotube (PVA-MWCNT) composite obtained by a freezing-thawing process. It comprises a MWCNT conduit, a PVA binder, and an ADH.function. The measurement of ethanol is based on the signal produced by P-nicotinamide adenine dinucleotide (NADH), the product of the enzymatic reaction. The homogeneity of the resulting biocomposite film was characterized by atomic force microscopy (AFM). The performance of the PVA-MWCNT-ADH biocomposite modified glassy carbon electrode was evaluated using cyclic voltarnmetry and amperometry in the presence of NADH and in the presence of ethanol. The ethanol content in standard solutions was determined and a sensitivity of 196 nA mM(-1), a linear range up to 1.5 mM, and a response time of about 8 s were obtained. These characteristics allowed its application for direct detection of ethanol in alcoholic beverages: beer, red wine, and spirit. (c) 2007 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiosensors & Bioelectronicsen_US
dc.relation.ispartofseriesBiosensors & Bioelectronics, Volume 22, Issue 12, Page(s) 3051-3056.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.bios.2007.01.005en_US
dc.subjectamperometric biosensoren_US
dc.subjectmultiwalled carbon nanotubesen_US
dc.subjectalcoholen_US
dc.subjectdehydrogenaseen_US
dc.subjectpoly(vinyl alcohol)en_US
dc.subjectethanol determinationen_US
dc.subjectcomposite electrochemical sensorsen_US
dc.subjectpva cryogel membraneen_US
dc.subjectglucose-oxidaseen_US
dc.subjectthin-filmen_US
dc.subjectmodified electrodesen_US
dc.subjectimmobilizationen_US
dc.subjectnanoparticlesen_US
dc.subjectoxidationen_US
dc.subjecthydrogelsen_US
dc.subjectnadhen_US
dc.titleAmperometric ethanol biosensor based on poly(vinyl alcohol)-multiwalled carbon nanotube-alcohol dehydrogenase biocompositeen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bios.2007.01.005zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
Appears in Collections:化學工程學系所
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