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標題: Electrodeposition of polypyrrole-multiwalled carbon nanotube-glucose oxidase nanobiocomposite film for the detection of glucose
作者: Tsai, Y.C.
Li, S.C.
Liao, S.W.
關鍵字: conducting polymer;polypyrrole;multiwalled carbon nanotubes;glucose;oxidase;nanobiocomposite;pyrolytic-graphite electrode;conducting-polymer;paste electrodes;biosensors;nanoparticles;composites;immobilization;oxidation;enzymes;design
Project: Biosensors & Bioelectronics
期刊/報告no:: Biosensors & Bioelectronics, Volume 22, Issue 4, Page(s) 495-500.
A nanobiocomposite film consisted of polypyrrole (PPy), functionalized multiwalled carbon nanotubes (cMWNTs), and glucose oxidase (GOx) were electrochemically synthesized by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of cMWNTs and GOx. Potentiostatic growth profiles indicate that the anionic cMWNTs is incorporated within the growing PPy-cMWNTs nanocomposite for maintaining its electrical neutrality. The morphology of the PPy-cMWNTs nanocomposite was characterized by scanning electron microscopy (SEM). The PPy-cMWNTs nanocomposite was deposited homogeneously onto glassy carbon electrode. The amperometric responses vary proportionately to the concentration of hydrogen peroxide at the PPy-cMWNTs nanocomposite modified electrode at an operating potential of 0.7 V versus Ag/AgCl (3 M). The results indicate that the electroanalytical PPy-cMWNTs-GOx nanobiocomposite film was highly sensitive and suitable for glucose biosensor based on GOx function. The GOx concentration within the PPy-cMWNTs-GOx nanobiocomposite and the film thickness are crucial for the performance of the glucose biosensor. The amperometric responses of the optimized PPy-cMWNTs-GOx glucose biosensor (1.5 mg mL(-1) GOx, 141 mC cm(-2) total charge) displayed a sensitivity of 95 nA mM(-1), a linear range up to 4 mM, and a response time of about 8 s. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 0956-5663
DOI: 10.1016/j.bios.2006.06.009
Appears in Collections:化學工程學系所

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