Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/41758
DC FieldValueLanguage
dc.contributor.authorTsai, Y.C.en_US
dc.contributor.author蔡毓楨zh_TW
dc.contributor.authorChen, S.Y.en_US
dc.contributor.authorLiaw, H.W.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:05:50Z-
dc.date.available2014-06-06T08:05:50Z-
dc.identifier.issn0925-4005zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/41758-
dc.description.abstractA composite of multiwalled carbon nanotubes-chitosan (MWCNT-CHIT) was used as a matrix for entrapment of lactate dehydrogenase (LDH) onto a glassy carbon electrode in order to fabricate amperometric biosensor. The homogeneity of the resulting multiwalled carbon nanotubes-chitosan-lactate dehydrogenase (MWCNT-CHIT-LDH) nanobiocomposite film was investigated by atomic force microscopy (AFM). It shows that the enzyme is homogeneously immobilized within MWCNT-CHIT-LDH. The inclusion of MWCNT within MWCNT-CHIT-LDH exhibits the abilities to raise the current responses, to decrease the electrooxidation potential of P-nicotinamide adenine dinucleotide, reduced form (NADH), and to prevent the electrode surface fouling. The influence of several experimental parameters such as applied potential, solution pH value, NAD(+) concentration, and enzyme loading was explored to optimize the electroanalytical performance of the biosensor. The optimized biosensor for the determination of lactate shows a sensitivity of 0.0083 AM(-1) cm(-2) and a response time of about 3 s. The proposed biosensor retained 65% of its original response after 7 days. (C) 2007 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationSensors and Actuators B-Chemicalen_US
dc.relation.ispartofseriesSensors and Actuators B-Chemical, Volume 125, Issue 2, Page(s) 474-481.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.snb.2007.02.052en_US
dc.subjectbiosensoren_US
dc.subjectamperometryen_US
dc.subjectmultiwalled carbon nanotubeen_US
dc.subjectlactateen_US
dc.subjectdehydrogenaseen_US
dc.subjectchitosanen_US
dc.subjectelectrocatalytic oxidationen_US
dc.subjectmodified electrodesen_US
dc.subjectelectrochemicalen_US
dc.subjectsensorsen_US
dc.subjectalcohol-dehydrogenaseen_US
dc.subjectpolyaniline filmsen_US
dc.subjectnadhen_US
dc.subjectoxidaseen_US
dc.subjectcompositeen_US
dc.subjectglucoseen_US
dc.subjectnanobiocompositeen_US
dc.titleImmobilization of lactate dehydrogenase within multiwalled carbon nanotube-chitos anhanocompo site for application to lactate biosensorsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.snb.2007.02.052zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:化學工程學系所
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