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標題: Preparation of multiwalled carbon nanotube-chitosan-alcohol dehydrogenase nanobiocomposite for amperometric detection of ethanol
作者: Lee, C.A.
Tsai, Y.C.
關鍵字: Multiwalled carbon nanotube;Alcohol dehydrogenase;Chitosan;Ethanol;biosensor;electrocatalytic oxidation;oxidase nanobiocomposite;modified;electrodes;gas-chromatography;nadh detection;biosensor;immobilization;glucose;beverages;graphite
Project: Sensors and Actuators B-Chemical
期刊/報告no:: Sensors and Actuators B-Chemical, Volume 138, Issue 2, Page(s) 518-523.
An amperometric biosensor for the determination of ethanol has been constructed using a nanobiocomposite film. This film is made up of a conduit of multiwalled carbon nanotubes (MWCNTs), a chitosan (CHIT) hinder, and an alcohol dehydrogenase (ADH) function. The MWCNT-CHIT-ADH nanobiocomposite film is cast oil a glassy carbon electrode. Homogeneity of the resulting nanobiocomposite film was investigated by field emission scanning electron microscopy. Electrocatalytic activity of the MWCNT-CHIT-ADH nanobiocomposite was determined using cyclic voltammetry and amperometry. Optimum conditions of the biosensor were experimentally determined through varying several important parameters. These included working potential, Solution pH value, NAD(+) concentration, and enzyme loading. The optimized biosensor shows a sensitivity of 0.1646 AM(-1) cm(-1), all apparent Michaelis-Menten constant of 0.38 mM, and a detection limit of 0.52 mu M. The applicability of the proposed biosensor based on MWCNT-CHIT-ADH nanobiocomposite was tested by the detection of ethanol in beer, red wine, and spirit. (C) 2009 Elsevier B.V. All rights reserved.
ISSN: 0925-4005
DOI: 10.1016/j.snb.2009.01.001
Appears in Collections:化學工程學系所

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