Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/42016
DC FieldValueLanguage
dc.contributor.authorChang, J.C.en_US
dc.contributor.author徐善慧zh_TW
dc.contributor.authorSu, H.L.en_US
dc.contributor.authorHsu, S.H.en_US
dc.contributor.author蘇鴻麟zh_TW
dc.date2008zh_TW
dc.date.accessioned2014-06-06T08:06:06Z-
dc.date.available2014-06-06T08:06:06Z-
dc.identifier.issn0142-9612zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/42016-
dc.description.abstractLabel of human bone mesenchymal stem cells with CdSe/ZnS quantum dots (QDs) had been demonstrated to impair cell functions and activities. In the present study, QDs delivered by two different routes, Pep-1-labeled QDs (LQ) and PolyFect transfected QDs (TQ), were utilized to assess the effects of delivery mechanisms on various cellular responses of the QDs-internalized human adipose-derived adult stem cells (hADAS). Examination of labeled cells by flow cytometry and laser scanning confocal microscopy showed that LQ had higher fluorescence intensity due to the cluster formation and their distribution in cytoplasma while TQ were preferentially accumulated at perinuclear regions. The fluorescence intensity of the LQ group was still higher than that of the TQ group at 28 days after labeling, though cellular LQ were partitioned after initial cell division. Pep-1 but not PolyFect delivery facilitated QDs to escape from lysosome degradation. Pep-1 delivery of QDs rescued the cells from the negative effects caused by the internalized QDs on cell proliferation and on the expressions of CD29 and CD105 as well as osteogenic and chondrogenic-associated lineage markers. The same effect was also observed in the expression of alkaline phosphatase activity, calcium deposition and secretion of chondrogenic matrices (GAG and collagen type II) in micromass culture. These indicated that Pep-1-delivered QDs may serve appropriately to track the hADAS employed in cell therapy/tissue engineering applications. The results also suggested that the endo-/lysosome degradation of QDs may depend on different surface coatings and critically influence the differentiation of hADAS. (c) 2007 Elsevier Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationBiomaterialsen_US
dc.relation.ispartofseriesBiomaterials, Volume 29, Issue 7, Page(s) 925-936.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.biomaterials.2007.10.046en_US
dc.subjectquantum dotsen_US
dc.subjectPep-1en_US
dc.subjecthuman adult adipose-derived stern cells (hADAS)en_US
dc.subjectlysosonneen_US
dc.subjectCD markersen_US
dc.subjectdifferentiationen_US
dc.subjectmammalian-cellsen_US
dc.subjectreceptor internalizationen_US
dc.subjectintracellular deliveryen_US
dc.subjectpenetrating peptidesen_US
dc.subjectnanoparticlesen_US
dc.subjectnanocrystalsen_US
dc.subjectendosomesen_US
dc.subjectvesiclesen_US
dc.subjecttrackingen_US
dc.subjectcarrieren_US
dc.titleThe use of peptide-delivery to protect human adipose-derived adult stem cells from damage caused by the internalization of quantum dotsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.biomaterials.2007.10.046zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:化學工程學系所
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.