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標題: Optimization of Enzymatic Synthesis of Cetyl 2-Ethylhexanoate by Novozym (R) 435
作者: Chen, H.C.
Kuo, C.H.
Chen, H.H.
Liu, Y.C.
Shieh, C.J.
關鍵字: Biocatalysis;Cetyl 2-ethylhexanoate;Lipase;Optimization;Response;surface methodology;Wax esters;lipase-catalyzed synthesis;surface methodology rsm;wax ester;water-content;alcoholysis;triolein;media;acid
Project: Journal of the American Oil Chemists Society
期刊/報告no:: Journal of the American Oil Chemists Society, Volume 88, Issue 12, Page(s) 1917-1923.
Waxes are esters obtained from long-chain fatty acids and long-chain alcohols which are biodegradable, biocompatible and nontoxic. Seafowl feather oil is a natural wax ester that exists on seafowl feathers. Cetyl 2-ethylhexanoate is the major ingredient of seafowl feather oil. Cetyl 2-ethylhexanoate is widely used in cosmetics as a base oil because of its lubricity, moisture retention and non-toxic properties. An optimal production of cetyl 2-ethylhexanoate by direct esterification of cetyl alcohol with 2-ethylhexanoic acid was developed using an immobilized lipase (Novozym(A (R)) 435) as a catalyst in n-hexane. Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) were employed to evaluate the effects of reaction time, reaction temperature, substrate molar ratio, and enzyme amount on the yield of cetyl 2-ethylhexanoate. The results show that reaction time, reaction temperature, substrate molar ratio, and enzyme amount have significant effects on the yield of the esterification reaction. On the basis of ridge-max analysis, the optimum conditions were as follows: a reaction time of 2.65 days, a reaction temperature of 56.18 A degrees C, a substrate molar ratio of 2.55:1, and an enzyme amount of 251.39%. The predicted and experimental values of molar conversion were 91.95 and 89.75 +/- A 1.06%, respectively.
ISSN: 0003-021X
DOI: 10.1007/s11746-011-1868-y
Appears in Collections:化學工程學系所

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