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標題: Production of all trans-beta-carotene by using impinging flow of supercritical carbon dioxide anti-solvent pulverization
作者: Shen, Yi-Chen
Chng, Lee-Muei
Wang, Yuan-Chuen
Shieh, Chwen-Jen
Lin, Kuo-Li
Hsu, Shih-Lan
Chou, Hong-Nong
Chieh-Ming, J.Changa
關鍵字: Dunaliella salina;Trans-beta-carotene;Column elution chromatography;Supercritical anti-solvent precipitation
Project: Journal of Chromatography A, Volume 1270, Page(s) 1–8.
This work investigated column elution chromatography coupled with supercritical anti-solvent precipitation
to produce carotenoid rich microsized particulates from microalgal Dunaliella salina species. The
extract contained carotenoids ranging from 61.3 mg/gsalina to 72.5 mg/gsalina using ultrasonic stirred ethyl
ether or tetrahydrofuran (THF) extraction. When 10 L of ethyl alcohol was employed to elute the THF
extract, purity of trans- -carotene is 823.6 mg/g with a recovery of 86.2%. It was found that the supercritical
anti-solvent of THF solution at 160 bar and 318 K produced powdered particulates with a purity
of carotenoids above 90%. Subsequently, a central composite response surface design method was used
to design supercritical anti-solvent precipitation of carotenoid-rich THF solution. This was accomplished
by increasing the pressure from 140 bar to 180 bar and the time from 40 min to 60 min at a feed flow rate
of 0.2 mL/min. A CO2 flow rate of 15 L/min and a temperature of 318 K were also used to determine the
effects on purity and recovery of trans- -carotene. The combined process produced micronized precipitates
with a mean particle size ranging from 3.5 m to 19 m and the purity of trans- -carotene attained
was 926.8 mg/g with a recovery of 54%.
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2012.10.036
Appears in Collections:化學工程學系所

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