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|標題:||Countercurrent flow of supercritical anti-solvent in the production of pure xanthophylls from Nannochloropsis oculata||作者:||Cho, Yueh-Cheng
Lin, Justin Chun-Te
|關鍵字:||Zeaxanthin;Column chromatography fractionation;Supercritical anti-solvent precipitation;Submicron-sized particulates||Project:||Journal of Chromatography A, Volume 1250, Page(s) 85–91.||摘要:||
This study examined pilot scaled elution chromatography coupled with supercritical anti-solvent precipitation
(using countercurrent flow) in generating zeaxanthin-rich particulates from a micro-algal species.
Ultrasonic agitated acetone extract subjected to column fractionation successfully yielded a fraction containing
349.4 mg/g of zeaxanthin with a recovery of 85%. Subsequently, supercritical anti-solvent (SAS)
precipitation of the column fraction at 150 bar and 343 K produced submicron-sized particulates with
a concentration of 845.5 mg/g of zeaxanthin with a recovery of 90%. Experimental results from a twofactor
response surface method SAS precipitation indicated that purity, mean size and morphology of the
precipitates were significantly affected by the flow type configuration, feed flow rate and injection time.
|Appears in Collections:||化學工程學系所|
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