Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/42097
DC FieldValueLanguage
dc.contributor.authorWu, Jiun-Yanen_US
dc.contributor.authorChen, Chih-Ien_US
dc.contributor.authorChen, Chi-Mingen_US
dc.contributor.authorLina, Chia-Chien_US
dc.contributor.authorKana, Shu-Chenen_US
dc.contributor.authorShieh, Chwen-Jenen_US
dc.contributor.authorLiua, Yung-Chuanen_US
dc.date2012zh_TW
dc.date.accessioned2014-06-06T08:06:12Z-
dc.date.available2014-06-06T08:06:12Z-
dc.identifier.issn1369-703Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/42097-
dc.description.abstractIn this study, three approaches for protein production were devised to enhance the efficiencies in yielding high purity protein via surface display system. A plasmid carrying enhanced green fluorescent protein (EGFP), an intein (INT) and ice nucleation protein (INP) was constructed to produce EGFP via surface display in Escherichia coli. To obtain high purity of the produced EGFP, several procedures, including osmotic shock, surfactant addition and cell disruption were employed. Among these approaches, the cell disruption method gave the highest EGFP purity by simply conducting several centrifugations. An EGFP yield of 63 mg/L with 97% purity was obtained. The result demonstrated that pure EGFP can be harvested only through centrifugation; no complicated processes or expensive equipment are required. This approach shows potential for the production of pure recombinant proteins in scale-up processes for biotechnological, academic and industrial uses.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical Engineering Journal, Volume 68, page(s) 12– 18.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.bej.2012.06.020en_US
dc.subjectProtein Purificationen_US
dc.subjectSurface displayen_US
dc.subjectInteinen_US
dc.subjectCentrifugationen_US
dc.subjectRecombinant DNAen_US
dc.titleCell disruption enhanced the pure EGFP recovery from an EGFP-intein-surface protein production system in recombinant E. colien_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bej.2012.06.020zh_TW
dc.contributor.catalogerWei-Chun Wangen_US
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
Appears in Collections:化學工程學系所
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