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標題: Development of the reversible PGA immobilization by using the immobilized metal ion affinity membrane
作者: Chenb, Chih-I
Ko, Yi-Miao
Lien, Wei-Ling
Lin, Yi-Hsuan
Li, I-Tsang
Chen, Chien-Hsun
Shieh, Chwen-Jen
Liu, Yung-Chuan
關鍵字: Immobilized metal affinity membrane;Enzyme immobilization;Penicillin G acylase;Regeneration process;Coordination immobilization
Project: Journal of Membrane Science, Volume 401– 402, page(s) 33– 39.
In this study, an immobilized metal ion affinity membrane (IMAM) was prepared and used to immobilize
penicillin G acylase (PGA). The stability of storage of the immobilized PGA membrane (IPM) was tested.
When stored in deionized water (DI) at 4 "C, only 19% residual activity of IPM was retained for 10 days.
However, when stored in 10 mM phosphate buffer (PB, pH 8) with 0.1% NaN3, the IPM can retain 99% of its
activity for a 10-day reaction and storage test. The feasibility of the IPM regeneration after a long period
of reaction was investigated. When using 100 mM EDTA as the stripping solution, the re-immobilized
capacity for PGA is only 52% of the original one after 4 regenerations. When the regeneration process was
modified by using the stripping solution (300 mM NaCl, 25 mM EDTA, 20 mM PB, pH 8) for 30 min, then
immersed in 0.5 M HCl and 0.5 M NaOH respectively for 10 min, the resulting IMAM can reserve 99% PGA
immobilized activity after 5 regenerations.
ISSN: 0376-7388
DOI: 10.1016/j.memsci.2012.01.022
Appears in Collections:化學工程學系所

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