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標題: Effect of membranes with various hydrophobic/hydrophilic properties on lipase immobilized activity and stability
作者: Chen, Guan-Jie
Kuo, Chia-Hung
Chen, Chih-I
Yu, Chung-Cheng
Shieh, Chwen-Jen
Liu, Yung-Chuan
關鍵字: Lipase;Covalent immobilization;Polyvinylidene fluoride;Hydrophobicity;Surface modification
Project: Journal of Bioscience and Bioengineering, Volume 113, Issue 2, page(s) 166–172.
In this study, three membranes: regenerated cellulose (RC), glass fiber (GF) and polyvinylidene fluoride (PVDF), were
grafted with 1,4-diaminobutane (DA) and activated with glutaraldehyde (GA) for lipase covalent immobilization. The
efficiencies of lipases immobilized on these membranes with different hydrophobic/hydrophilic properties were compared.
The lipase immobilized on hydrophobic PVDF-DA-GA membrane exhibited more than an 11-fold increase in activity compared
to its immobilization on a hydrophilic RC-DA-GA membrane. The relationship between surface hydrophobicity and
immobilized efficiencies was investigated using hydrophobic/hydrophilic GF membranes which were prepared by grafting a
different ratio of n-butylamine/1,4-diaminobutane (BA/DA). The immobilized lipase activity on the GF membrane increased
with the increased BA/DA ratio. This means that lipase activity was exhibited more on the hydrophobic surface. Moreover, the
modified PVDF-DA membrane was grafted with GA, epichlorohydrin (EPI) and cyanuric chloride (CC), respectively. The lipase
immobilized on the PVDF-DA-EPI membrane displayed the highest specific activity compared to other membranes. This
immobilized lipase exhibited more significant stability on pH, thermal, reuse, and storage than did the free enzyme. The
results exhibited that the EPI modified PVDF is a promising support for lipase immobilization.
ISSN: 1389-1723
DOI: 10.1016/j.jbiosc.2011.09.023
Appears in Collections:化學工程學系所

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