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標題: Cytotoxicity and drug release behavior of PNIPAM grafted on silica-coated iron oxide nanoparticles
作者: Lien, Yi-Hsin
Wu, Tzong-Ming
Wu, Jhao-Huei
Liao, Jiunn-Wang
關鍵字: Drug carrier;Adsorption;MTT;Cell line;Cytotoxicity;Biocompatible;Nanomedicine
出版社: Springer Netherlands
Project: Journal of Nanoparticle Research, Volume 13, Issue 10, Page(s) 5065-5075.
The nanoparticles containing thermosensitive and magnetic properties were investigated for their potential use as a novel drug carrier for targeted and controlled release drug delivery system. These thermosensitive and magnetic nanoparticles were prepared by grafting thermosensitive poly (N-isopropylacrylamide) (PNIPAM) on the surface of silica (SiO2)-coated Fe3O4 nanoparticles with the particle size of 18.8 1.6 nm. Adsorption and desorption behavior of bovine serum albumin (BSA) on the surface of PNIPAM-grafted SiO2/Fe3O4 nanoparticles was studied, and the results indicated that these nanoparticles were able to absorb protein at temperature above the lower critical solution temperature (LCST) and to be desorbed below the LCST. Cytotoxicity studies conducted on Chinese hamster ovary (CHO-K1) cells using methyl tetrazolium (MTT) assays revealed that cell viability of 1 mg/mL PNIPAM-grafted nanoparticles was slightly decreased after 24 h of incubation as compared to the lower concentration of nanoparticles. Furthermore, the concentration of 0.5 mg/mL PNIPAM-grafted nanoparticles was totally biocompatible for 48 h, but had low cytotoxicity after 72 h of incubation. These PNIPAM-grafted nanoparticles did not induce morphological change in their cellularity after exposure for 24 and 108 h. These results demonstrate that PNIPAM-grafted nanoparticles are biocompatible and have potential use as drug carriers.
ISSN: 1388-0764
DOI: 10.1007/s11051-011-0487-8
Appears in Collections:材料科學與工程學系

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