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Optimization of Enzymatic Bio-Fuel Cell for Immobilization of Glucose Oxidase on Chitosan Coated Carbon Cloth
|關鍵字:||酵素型生物燃料電池;Enzymatic Bio-Fuel Cell;共價鍵結法;反應曲面法;Covalent ImmobilizingResponse Surface Methodology||出版社:||精密工程學系所||引用:|| S. Calabrese Barton, J. Gallaway, and P. Atanassov, “Enzymatic Biofuel Cells for Implantable and Microscale Devices,” ChemInform, vol. 35, no. 51, pp. no-no, 2004.  F. Davis, and S. P. J. Higson, “Biofuel cells—Recent advances and applications,” Biosensors and Bioelectronics, vol. 22, no. 7, pp. 1224-1235, 2007.  M. H. Osman, A. A. Shah, and F. C. Walsh, “Recent progress and continuing challenges in bio-fuel cells. Part I: Enzymatic cells,” Biosensors and Bioelectronics, vol. 26, no. 7, pp. 3087-3102, 2011.  A. Sanchez, J. L. del Rı́o, F. Valero et al., “Continuous enantioselective esterification of trans-2-phenyl-1-cyclohexanol using a new Candida rugosa lipase in a packed bed bioreactor,” Journal of Biotechnology, vol. 84, no. 1, pp. 1-12, 2000.  陳國誠, “生物固定化技術與產業應用,” 茂昌圖書有限公司, pp. 125-164, 1990.  鄭慈千, “固定化酵素生產果寡糖與半乳寡糖,” 生物工程學系(所), 大同大學, 台北市, 2005.  S. F. D''Souza, N. Agric., and B. Division, “Immobilized enzymes in bioprocess,” Current Science, vol. 77, no. 1, pp. 69-79, 1999.  陳. 洪哲潁, “回應曲面實驗設計法在微生物酵素生產上之應用,” 化工專論, vol. 39, no. 2, pp. 3-18, 1992.  M. N. V. Ravi Kumar, “A review of chitin and chitosan applications,” Reactive and Functional Polymers, vol. 46, no. 1, pp. 1-27, 2000.  徐世昌, “生物性高分子—「幾丁質及幾丁聚醣」之介紹與應用,” 化工資訊, vol. 2, pp. 36-45, 2001.  趙恩中, “以酪胺酸酵素修飾幾丁聚醣 應用於化工程序之研究,” 化學工程與材料工程研究所, 國立中央大學, 桃園縣, 2004.  C. Fuentes-Albarran, A. Del Razo, K. Juarez et al., “Influence of NaCl, Na2SO4 and O2 on power generation from microbial fuel cells with non-catalyzed carbon electrodes and natural inocula,” Solar Energy, vol. 86, no. 4, pp. 1099-1107, 2012.  沈明來, “試驗設計學,” 九州出版社, pp. 612-655, 2010.  I. Ivanov, T. Vidaković-Koch, and K. Sundmacher, “Direct hybrid glucose–oxygen enzymatic fuel cell based on tetrathiafulvalene–tetracyanoquinodimethane charge transfer complex as anodic mediator,” Journal of Power Sources, vol. 196, no. 22, pp. 9260-9269, 2011.  J. Shim, G.-Y. Kim, and S.-H. Moon, “Covalent co-immobilization of glucose oxidase and ferrocenedicarboxylic acid for an enzymatic biofuel cell,” Journal of Electroanalytical Chemistry, vol. 653, no. 1–2, pp. 14-20, 2011.  B. C. Kim, X. Zhao, H.-K. Ahn et al., “Highly stable enzyme precipitate coatings and their electrochemical applications,” Biosensors and Bioelectronics, vol. 26, no. 5, pp. 1980-1986, 2011.  C. Chen, L. Wang, Y. Tan et al., “High-performance amperometric biosensors and biofuel cell based on chitosan-strengthened cast thin films of chemically synthesized catecholamine polymers with glucose oxidase effectively entrapped,” Biosensors and Bioelectronics, vol. 26, no. 5, pp. 2311-2316, 2011.  劉俊良, “可攜式酵母菌微生物燃料電池系統與發電特性研究,” 精密工程所碩士論文, 中興大學, 台中市, 2008.  K. Rabaey, and W. Verstraete, “Microbial fuel cells: novel biotechnology for energy generation,” Trends in Biotechnology, vol. 23, no. 6, pp. 291-298, 2005.  SONY. "http://www.sony.com/index.php."  H. Sakai, T. Nakagawa, Y. Tokita et al., “A high-power glucose/oxygen biofuel cell operating under quiescent conditions,” Energy & Environmental Science, vol. 2, no. 1, pp. 133-138, 2009.  B. E. Logan, B. Hamelers, R. Rozendal et al., “Microbial Fuel Cells: Methodology and Technology,” Environmental Science & Technology, vol. 40, no. 17, pp. 5181-5192, 2006/09/01, 2006.  K. Min, J. H. Ryu, and Y. J. Yoo, “Mediator-free Glucose/O2 Biofuel Cell Based on a 3-Dimensional Glucose Oxidase/SWNT/Polypyrrole Composite Electrode,” Biotechnology and Bioprocess Engineering, vol. 15, pp. 371-375, 2010.  M. Fischback, K. Y. Kwon, I. Lee et al., “Enzyme precipitate coatings of glucose oxidase onto carbon paper for biofuel cell applications,” Biotechnology and Bioengineering, vol. 109, no. 2, pp. 318-324, 2012.  A. Zebda, C. Gondran, A. Le Goff et al., “Mediatorless high-power glucose biofuel cells based on compressed carbon nanotube-enzyme electrodes,” Nature Communication, vol. 2, pp. 370, 2011.||摘要:||
This study presents a high-performance biofuel cell based on the covalent immobilizing of glucose oxidase (GOx) on chitosan coated carbon cloth as an anodic catalyst. The chitosan was coated by the coagulation of an aqueous solution of chitosan on the carbon cloth surface. The N-(3-dimethylaminopropyl)-N''-ethylcarbodiimide (EDC) and N-hydroxysuccinimide (NHS) was used as coupling agents for GOx immobilization. The response surface methodology (RSM) and Box-Behnken design were employed to search the optimal immobilization conditions and understand the significance of the factors affecting the immobilized GOx activity. The results indicated that the pH, and the enzyme/support ratio are the statistically significant factors for GOx immobilization. In the ridge max analysis, the optimal immobilization conditions include a reaction time of 50 min, a pH of 5.9, and an enzyme/support ratio of 3 (w/w). Under the optimal condition, the predicted and the experimental immobilized GOx activities were 34.42�1.07 and 33.50�0.92 U/g-support, respectively. Based on the regression model, the carbon cloths with various GOx activities were prepared, and the GOx activity effect on the power density generated from the biofuel cell was investigated. The power density was increased with GOx activity, and the maximum power 1.672 mW/cm2 was obtained at a cell voltage of 0.44 V.
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