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標題: Tepidimonas taiwanensis sp nov., a novel alkaline-protease-producing bacterium isolated from a hot spring
作者: Chen, T.L.
Chou, Y.J.
Chen, W.M.
Arun, B.
Young, C.C.
關鍵字: Tepidimonas taiwanensis;beta-Proteobacteria;hot spring;alkaline;protease;taxonomy;deoxyribonucleic-acid;gen.-nov.;extremophiles;culture;enzymes
Project: Extremophiles
期刊/報告no:: Extremophiles, Volume 10, Issue 1, Page(s) 35-40.
The bacterial strain designated I1-1(T) was isolated from a hot spring located in the Pingtung area, Southern Taiwan. Cells of this organism were Gram reaction negative rods, motile by a single polar flagellum. Optimum conditions for growth were 55 degrees C and pH 7. Strain I1-1(T) grew well in lower nutrient media such LIS 5-10% Luria-Bertani broth, and its extracellular products expressed alkaline protease activity. The 16S rRNA gene sequence analysis indicates that strain I1-1(T) is a member of beta-Proteobacteria. On the basis of a phylogenetic analysis of 16S rDNA sequences, DNA-DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well Lis fatty acid compositions, the organism belonged to the genus Tepidimonas and represented a novel species within this genus. The predominant cellular fatty acids of strain I1-1(T) were 16:0 (about 41%), 18:1 omega 7c (about 13%) and summed feature 3 [16:1 w7c or 15:0 iso 2OH or both (about 26%)]. Its DNA base ratio, was 68.1 mol%. We propose to classify strain I1-1(T) (=BCRC 17406(T)= LMG 22826(T)) as Tepidimonas taiwanensis sp. nov.
ISSN: 1431-0651
DOI: 10.1007/s00792-005-0469-9
Appears in Collections:土壤環境科學系

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