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|標題:||Flectobacillus roseus sp nov., isolated from freshwater in Taiwan||作者:||Sheu, S.Y.
|關鍵字:||deoxyribonucleic-acid;sequence alignment;genus microcyclus;comb-nov;bacterium;glomeratus;proposal;trees||Project:||International Journal of Systematic and Evolutionary Microbiology||期刊/報告no：:||International Journal of Systematic and Evolutionary Microbiology, Volume 59, Page(s) 2546-2551.||摘要:||
Two Gram-negative, aerobic, pale pink/rose-coloured, rod-shaped, non-motile bacterial strains, designated GFA-11(T) and RG-4, were isolated from a freshwater fish culture pond and a freshwater spring sample, respectively. Based on characterization by using a polyphasic approach, the two strains showed highly similar phenotypic, physiological and genetic characteristics. They shared 99.9% 16S rRNA gene sequence similarity and 89-94% DNA-DNA relatedness, suggesting that they represent a single genomic species. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains GFA-11(T) and RG-4 formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Flectobacillus within the family Flexibacteraceae. Their closest neighbours were Flectobacillus major DSM 103(T) (97.7 % 16S rRNA gene sequence similarity) and Flectobacillus lacus CL-GP79(T) (95.9%). Levels of DNA-DNA relatedness between the two novel strains and the type strains of F. major and F. lacus were less than 70%. The results of physiological and biochemical tests allowed clear phenotypic differentiation of strains GFA-11(T) and RG-4 from recognized members of the genus Flectobacillus. The predominant fatty acid constituents of strain GFA-11(T) were C(16:1)omega 5c (40.2% of the total) and iso-C(15:0) (15.0%). The DNA G + C content of strain GFA-11(T) was 39.7 mol%. On the basis of 16S rRNA gene sequence analysis and chemotaxonomic and physiological data, strains GFA-11(T) and RG-4 are considered to represent a novel species of the genus Flectobacillus, for which the name Flectobacillus roseus sp. nov. is proposed. The type strain is GFA-11(T) (=BCRC 17834(T)=LMG 24501(T)).
|Appears in Collections:||土壤環境科學系|
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