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|標題:||Gordonia malaquae sp nov., isolated from sludge of a wastewater treatment plant||作者:||Yassin, A.F.
|關鍵字:||thin-layer;actinomycete;chromatography;proposal;genus;acid;classification;identification;tsukamura;nocardiae||Project:||International Journal of Systematic and Evolutionary Microbiology||期刊/報告no：:||International Journal of Systematic and Evolutionary Microbiology, Volume 57, Page(s) 1065-1068.||摘要:||
The taxonomic status of a bacterial isolate from the sludge of a wastewater treatment plant was characterized by using a polyphasic taxonomic approach. Chemotaxonomic investigations revealed the presence of cell-wall chemotype IV, short-chain mycolic acids that co-migrated with those extracted from members of the genus Gordonia, fatty acids C(16:0) and C(18:0) (found by pyrolysis gas chromatography) and a dihydrogenated menaquinone with nine isoprene units [MK-9(H2)] as the predominant menaquinone. The genus assignment was confirmed by 16S rRNA gene sequencing. Comparative analysis of the 16S rRNA gene sequence showed that the novel isolate constitutes a hitherto unknown subline within the genus Gordonia, displaying 95.9 to 97.6% gene sequence similarity to the recognized species of the genus. The novel isolate was distinguished from the type strains of phylogenetically related species by using a set of phenotypic features. The genotypic and phenotypic data show that the new strain merits classification as a novel species of the genus Gordonia, for which the name Gordonia malaquae sp. nov. is proposed. The type strain is IMMIB WWCC-22(T) (= DSM 45064(T)= CCUG 53555(T)).
|Appears in Collections:||土壤環境科學系|
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