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|標題:||Novosphingobium soli sp. nov., isolated from soil||作者:||Kampfer, P.
|關鍵字:||genus sphingomonas;lipid-composition;activated-sludge;bacterium;identification;proposal;water;lake||Project:||International Journal of Systematic and Evolutionary Microbiology||期刊/報告no：:||International Journal of Systematic and Evolutionary Microbiology, Volume 61, Page(s) 259-263.||摘要:||
A yellow-pigmented, Gram-negative, rod-shaped, non-spore-forming bacterium, strain CC-TPE-1(T), was isolated from oil-contaminated soil near an oil refinery located in Kaohsiung County, Taiwan. 16S rRNA gene sequence analysis of strain CC-TPE-1(T) showed highest sequence similarity to Novosphingobium naphthalenivorans TUT562(T) (98.1%), N. panipatense SM16(T) (97.9 %) and N. mathurense SM117(T) (97.6%) and lower (< 97 %) sequence similarity to all other Novosphingobium species. DNA DNA hybridizations of strain CC-TPE-1(T) with N. naphthalenivorans DSM 18518(T), N. panipatense SM16(T) and N. mathurense SM117(T) showed low relatedness of 30% (reciprocal 35%), 29.1 % (reciprocal 30.6%) and 35% (reciprocal 23.6%), respectively. The major respiratory quinone was ubiquinone Q-10, the predominant fatty acid was C(18:1)omega 7c (49.9%) and three 2-hydroxy fatty acids, C(14:0) 2-OH (8.2%), C(15:0) 2-OH (2.45%) and C(16:0) 2-OH (1.05%), were detected. Polar lipids consisted mainly of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyl-dimethylethanolamine, two sphingoglycolipids, phosphatidylmonomethylethanolamine and several unidentified lipids, and a yellow pigment was also detected. The polyamine pattern contained the single major compound spermidine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis and polyamine, ubiquinone, polar lipid and fatty acid compositions revealed that strain CC-TPE-1(T) represents a novel species of the genus Novosphingobium, for which we propose the name Novosphingobium soli sp. nov., with the type strain CC-TPE-1(T) (=DSM 22821(T) =CCM 7706(T) =CCUG 58493(T)).
|Appears in Collections:||土壤環境科學系|
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