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標題: Indigenous rhizobia associated with native shrubby legumes in Taiwan
作者: Hung, M.H.
Bhagwath, A.A.
Shen, F.T.
Devasya, R.P.
Young, C.C.
關鍵字: shrubby legumes;rhizobia;PCR-RFLP;165 rRNA;nitrogen fixation;root-nodules;ralstonia-taiwanensis;nitrogen-fixation;sp nov.;lipopolysaccharide;proteobacteria;diversity;strains;bradyrhizobia;nodulation
Project: Pedobiologia
期刊/報告no:: Pedobiologia, Volume 49, Issue 6, Page(s) 577-584.
Root-nodulating bacteria were isolated and characterized from seven native shrubby legumes growing in Taiwan. Phenotypic characteristics measured included growth rates in various media, colony morphology, and tolerances to extremes of temperature, salt and pH. The isolates were very diverse phenotypically. Among the 83 isolates that were screened, the majority were fast-growing rhizobia. Twenty eight strains tolerated high concentration of salt (4.5% NaCl) and grew well between temperatures of 37 and 45 degrees C. The majority of the strains also tolerated extreme pH in their medium from 3.5 to 12. All strains formed nitrogen fixing nodules, and the highest activity was detected in the legume Hedysarum crinita L. PCR restriction fragment length polymorphism (PCR-RFLP) and sequencing of the small subunit ribosomal RNAs revealed that the majority of the isolates belonged to the genera Rhizobium, Bradyrhizobium and Agrobacterium. Only a single strain represented the genus Sinorhizobium. In addition, a strain related to Burkholderia from the beta-class of the Proteobacteria (CC-CC-5) was found within nodules of the legume Catenaria caudatum. The study contributes to the understanding of symbiotic nitrogen fixation in selected wild legumes that are native to Taiwan and provides insights into the distribution of nodulating and nitrogen-fixing bacteria from other distinct lineages. (c) 2005 Elsevier GmbH. All rights reserved.
ISSN: 0031-4056
DOI: 10.1016/j.pedobi.2005.06.002
Appears in Collections:土壤環境科學系

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