Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44778
DC FieldValueLanguage
dc.contributor.authorShen, C.T.en_US
dc.contributor.author楊秋忠zh_TW
dc.contributor.authorChen, P.Y.en_US
dc.contributor.authorWu, J.J.en_US
dc.contributor.authorLee, T.M.en_US
dc.contributor.authorHsu, S.L.en_US
dc.contributor.authorChang, C.M.J.en_US
dc.contributor.authorYoung, C.C.en_US
dc.contributor.authorShieh, C.J.en_US
dc.contributor.author張傑明zh_TW
dc.date2011zh_TW
dc.date.accessioned2014-06-06T08:13:15Z-
dc.date.available2014-06-06T08:13:15Z-
dc.identifier.issn0896-8446zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44778-
dc.description.abstractThis work investigated the changes in content of algal zeaxanthin in submicronized precipitates generated from the supercritical anti-solvent (SAS) process of extracting microalgae Nannochloropsis oculata. Following a reverse phase elution chromatography, the particulates were successfully generated from feed solutions containing zeaxanthin that ranged from 0.4 to 0.8 mg/mL by a SAS process. The precipitation condition was set at 323 K and pressures ranged from 10 to 20 MPa. Experimental results of a three-factor center composite response surface method for the SAS process indicated that the size of the precipitates was significantly affected by the flow rate of carbon dioxide. The purity of zeaxanthin increased with increasing solvent flow rate and with reducing solution concentration. The recovery of zeaxanthin and the morphology of the precipitates was also examined. The content of zeaxanthin in sub-micronsized precipitates increased from 485.9(48.6%) to 673.7 mg/g (67.4%). This work demonstrates that elution chromatography coupled with a SAS process is an environmentally benign method to recover anti-tyrosinase zeaxanthin from Nannochloropsis oculata as well as to generate submicrosized precipitates of the purest zeaxanthin from algal solutions. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Supercritical Fluidsen_US
dc.relation.ispartofseriesJournal of Supercritical Fluids, Volume 55, Issue 3, Page(s) 955-962.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.supflu.2010.10.003en_US
dc.subjectNannochloropsis oculataen_US
dc.subjectZeaxanthinen_US
dc.subjectElution chromatographyen_US
dc.subjectSubmicrosize particlesen_US
dc.subjectSupecritical anti-solvent precipitationen_US
dc.subjectmacular degenerationen_US
dc.subjectbeta-caroteneen_US
dc.subjectantisolvent precipitationen_US
dc.subjectbrazilian propolisen_US
dc.subjectphotoprotectionen_US
dc.subjectparticlesen_US
dc.subjectco2en_US
dc.subjectextractionen_US
dc.subjectfluidsen_US
dc.subjectplantsen_US
dc.titlePurification of algal anti-tyrosinase zeaxanthin from Nannochloropsis oculata using supercritical anti-solvent precipitationen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.supflu.2010.10.003zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:土壤環境科學系
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