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標題: Azospirillum formosense sp. nov., a diazotroph from agricultural soil
作者: Lin, Shih-Yao
Shen, Fo-Ting
Young, Li-Sen
Zhu, Zhi-Long
Chen, Wen-Ming
Young, Chiu-Chung
Project: International Journal of Systematic and Evolutionary Microbiology, Volume 62, Page(s) 1185–1190.
A Gram-negative, spiral or rod-shaped, non-spore-forming diazotrophic bacterium, designated
CC-Nfb-7T, was isolated from agricultural soil in Yunlin County, Taiwan. 16S rRNA gene
sequence analysis showed that strain CC-Nfb-7T was most closely related to Azospirillum
brasilense DSM 1690T (97.4% 16S rRNA gene sequence similarity), Azospirillum rugosum
IMMIB AFH-6T (96.8 %) and Azospirillum oryzae JCM 21588T (96.6 %); ,96.5% 16S rRNA
gene sequence similarity was found with all other members of the genus Azospirillum. DNA–DNA
relatedness between strain CC-Nfb-7T and A. brasilense DSM 1690T, A. rugosum DSM 19657T
and A. oryzae JCM 21588T was 38.9, 30.1 and 31.8 %, respectively. The respiratory quinone was
ubiquinone Q-10. The major fatty acids were summed feature 8 (consisting of C18 : 1v7c and/or
C18 : 1v6c), summed feature 3 (consisting of C16 : 1v7c and/or C16 : 1v6c), summed feature 2
(consisting of C14 : 0 3-OH and/or iso-C16 : 1 I), C16 : 0, C18 : 0 2-OH and C16 : 0 3-OH. The polar
lipids consisted mainly of phosphatidylglycerol, phosphatidylcholine and one unidentified
phospholipid. Furthermore, moderate amounts of phosphatidylethanolamine, phosphatidyldimethylethanolamine
and one unidentified aminophospholipid were also detected. Strain
CC-Nfb-7T could be distinguished from members of phylogenetically related species by
differences in phenotypic properties. On the basis of morphological, chemotaxonomic and
phylogenetic data, strain CC-Nfb-7T represents a novel species within the genus Azospirillum, for
which we propose the name Azospirillum formosense sp. nov. The type strain is CC-Nfb-7T
(5BCRC 80273T5JCM 17639T5DSM 24137T).
ISSN: 1466-5026
DOI: 10.1099/ijs.0.030585-0
Appears in Collections:土壤環境科學系

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