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標題: Sphingomonas formosensis sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from agricultural soil
作者: Lin, Shih-Yao
Shen, Fo-Ting
Lai, Wei-An
Zhu, Zhi-Long
Chen, Wen-Ming
Chou, Jui-Hsing
Lin, Zih-Yu
Young, Chiu-Chung
Project: International Journal of Systematic and Evolutionary Microbiology, Volume 62, Page(s) 1581–1586.
In the present study, a yellow-pigmented, Gram-negative, short rod-shaped novel bacterium that
was capable of degrading a wide range of polycyclic aromatic hydrocarbons (naphthalene,
phenanthrene and pyrene) was isolated from agricultural soil located in Yunlin County, Taiwan.
Comparative 16S rRNA gene sequence analysis positioned the novel strain in the genus
Sphingomonas as an independent lineage adjacent to a subclade containing Sphingomonas
fennica K101T, Sphingomonas histidinilytica UM2T, Sphingomonas wittichii RW1T and
Sphingomonas haloaromaticamans A175T. 16S rRNA gene sequence analysis of strain CC-Nfb-
2T showed highest sequence similarity to S. fennica K101T (96.2 %), S. histidinilytica UM2T
(96.1 %), S. wittichii RW1T (95.9 %), S. haloaromaticamans A175T (95.7 %), and Sphingobium
ummariense RL-3T (94.7 %); lower sequence similarities were observed with strains of all other
Sphingomonas species. The strain contained phosphatidylcholine, phosphatidylethanolamine,
phosphatidylglycerol, sphingoglycolipid and diphosphatidylglycerol. The predominant fatty acids
were summed feature 8 (C18 : 1v7c and/or C18 : 1v6c) C16 : 0 and 11-methyl C18 : 1v7c; C14 : 0 2-
OH was the major 2-hydroxy fatty acid. Previously, these lipids have been found to be
characteristic of members of the genus Sphingomonas. The serine palmitoyl transferase gene
(spt) was also detected and sphingolipid synthesis was confirmed. The predominant isoprenoid
quinone system was ubiquinone (Q-10) and the isolate contained sym-homospermidine as the
major polyamine. The DNA G+C content of the isolate was 62.8�0.8 mol%. On the basis of
chemotaxonomic, phenotypic and phylogenetic data, strain CC-Nfb-2T represents a novel
species within the genus Sphingomonas, for which the name Sphingomonas formosensis sp. nov.
is proposed; the type strain is CC-Nfb-2T (5BCRC 80272T5DSM 24164T).
ISSN: 1466-5026
DOI: 10.1099/ijs.0.034728-0
Appears in Collections:土壤環境科學系

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