Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44865
DC FieldValueLanguage
dc.contributor.authorT.A.Linen_US
dc.contributor.authorC.H.Chenen_US
dc.contributor.authorL.Y.Sungen_US
dc.contributor.authorM.G.Carteren_US
dc.contributor.authorY.E.Chenen_US
dc.contributor.authorF.Duen_US
dc.contributor.authorJ.C.Juen_US
dc.contributor.authorJ.Xuen_US
dc.contributor.otherNational Chung Hsing University,Department of Animal Scienceen_US
dc.contributor.other國立中興大學動物科學系zh_TW
dc.date2011-3zh_TW
dc.date.accessioned2014-06-06T08:13:58Z-
dc.date.available2014-06-06T08:13:58Z-
dc.identifier.issn0093-691Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44865-
dc.description.abstractThe objective was to determine cryotolerance of in vitro cultured rabbit embryos to the open-pulled straw (OPS) method. Overall, 844 rabbit embryos at pronuclear, 2- to 4-cell, 8-cell, and morula/blastocyst stages were vitrified, and >= 1 mo later, were sequentially warmed, rehydrated, and subjected to continuous culture (n = 691) or embryo transfer (ET, n = 153). Embryos vitrified at the 8-cell stage or beyond had greater survival, expanded blastocyst and hatched blastocyst rates in vitro, and better term development than those vitrified at earlier stages. The 8-cell group had 70.1% expanded blastocysts, 63.7% hatched blastocysts, and 25.7% term development, as compared to 1.5-17.7%, 1.5-4.3% and 2.8-3.7% in the pronuclear, 2-cell and 4-cell embryos, respectively (P < 0.05). The expanded and hatched blastocyst rates in vitrified morula/blastocyst post-warming were higher than that in the 8-cell group; however, their term development after ET was similar (8-cell vs morula/blastocyst: 25.7 vs 19.4%, P > 0.05). Development after ET was comparable between vitrified-warmed embryos and fresh controls at 8-cell and morula/blastocyst stages (19.4-25.7 vs 13.7-26.6%, P > 0.05). For embryos at pronuclear or 2- to 4-cell stages, however, term rates were lower in the vitrified-warmed (2.8-3.7%) than in fresh controls (28.6-35.6%, P < 0.05). Therefore, cultured rabbit embryos at various developmental stages had differential crytolerance. Under the present experimental conditions, the 8-cell stage appeared to be the critical point for acquiring cryotolerance. We inferred that for this OPS cryopreservation protocol, rabbit embryos should be vitrified no earlier than the 8-cell stage, and stage-specific protocols may be needed to maximize embryo survival after vitrification and re-warming. (C) 2011 Elsevier Inc. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationTheriogenology, Volume 75, Issue 4, Page(s) 760-768.en_US
dc.relation.isversionofTheriogenologyen_US
dc.subjectCryotoleranceen_US
dc.subjectOpen-pulled strawen_US
dc.subjectRabbiten_US
dc.subjectVitrificationen_US
dc.subjectfull-term developmenten_US
dc.subjectcell nuclear transferen_US
dc.subjectmouse oocytesen_US
dc.subjecttransgenicen_US
dc.subjectrabbitsen_US
dc.subjectops vitrificationen_US
dc.subjecthuman blastocystsen_US
dc.subjectporcine embryosen_US
dc.subjectethylene-glycolen_US
dc.subjectcryopreservationen_US
dc.subjectsurvivalen_US
dc.titleOpen-pulled straw vitrification differentiates cryotolerance of in vitro cultured rabbit embryos at the eight-cell stageen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.theriogenology.2010.10.018zh_TW
dc.contributor.catalogerMiao-zhen Luoen_US
item.languageiso639-1en_US-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
Appears in Collections:動物科學系
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