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標題: Differential expression of porcine testis proteins during postnatal development
作者: Huang, San-Yuan
Lin, Jyh-Hung
Teng, Shih-Hua
Sun, H.Sunny
Chen, Yu-Hui
Chen, Hsin-Hsin
Liao, Jun-Yi
Chung, Meng-Ting
Chen, Ming-Yu
Chuang, Chin-kai
Lin, En-Chung
Huang, Mu-Chiou
關鍵字: Pig;Testis;Protein expression;Proteome;Postnatal development;spermatogenesis-related genes;ipg strip prefractionation;lc-ms/ms;identification;proteomic analysis;mouse spermatogenesis;gel-electrophoresis;round spermatids;rat testis;mice;testosterone
Project: Animal Reproduction Science, Volume 123, Issue 3-4, Page(s) 221-233.
The development of the testes includes changes in cell morphology and endocrine levels that are essential for the maturation of males. A large number of novel proteins are expressed throughout testis development and play important roles in spermatogenesis. Differences in protein expressions during the development of porcine testes have not been systematically studied. The purpose of this study was to investigate differential protein expression in porcine testes during postnatal development. Testes from four pigs each at 1 wk, 3 mo, and 1 yr of age were used for a proteomic analysis. Expression levels of 264 protein spots were quantified using the Melanie 3 software. In total, 108 protein spots showed more than 2-fold differences (P < 0.05) among developmental stages, and 90 of them were successfully identified by mass spectrometry. The proteins were sorted based on whether the expression levels increased with age (36.1%), decreased with age (38.0%), or fluctuated among different developmental stages (25.9%). In total. 69 unique gene products were further classified according to their gene ontology annotations. A majority of the proteins are organelle proteins (41%) with the nucleus and mitochondria being the main organelles. About 45% of the proteins have a protein binding domain and are likely involved in protein-protein interactions. Finally, a large proportion of these differentially expressed proteins are involved in cellular (25%) and metabolic (22%) processes. Identifying these differentially expressed proteins should be valuable for exploring developmental biology and the pathology of male reproduction. (C) 2010 Elsevier B.V. All rights reserved.
ISSN: 0378-4320
DOI: 10.1016/j.anireprosci.2010.11.015
Appears in Collections:動物科學系

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