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|標題:||Butyrolactone I reversibly alters nuclear configuration, periooplasmic microtubules and development of porcine oocytes||作者:||Weng, Y.C.
|關鍵字:||cdc2 kinase;germinal vesicle;microtubule;oocyte;pig;cyclin-dependent kinases;germinal vesicle stage;pig oocytes;bovine;oocytes;chromosome condensation;meiotic inhibition;vitro maturation;mouse oocytes;cdc2 kinase;roscovitine||Project:||Theriogenology||期刊/報告no：:||Theriogenology, Volume 67, Issue 3, Page(s) 509-519.||摘要:||
In the present study, we investigated the effects of specific cdc2 kinase inhibitor, butyrolactone I (BL 1) on the prevention of germinal vesicle breakdown, changes of microtubular structures, and development of porcine oocytes after removal of the drug. In Experiment 1, cumulus-oocyte complexes (COCs) were cultured (44 h) in NCSU-23 medium containing different concentrations of BL I. The percentages of oocytes remaining at GV stage were 0, 0, 32, 80, and 84% (P < 0.05), and the maturation rates were 86, 63, 30, 0, and 0% (P < 0.05) for oocytes treated with 0, 10, 20, 40, and 80 mu M of BL I, respectively. When oocytes were released from BL I incubation (Experiment 2) and cultured for an additional 44 h, 79, 84, and 83% of oocytes resumed meiosis, but only 52, 38 and 17% of oocytes reached normal metaphase 11 (MII) stage in the groups treated with 20, 40 and 80 mu M BL 1, respectively. In Experiments 3-5, reversibility and development of oocytes and embryos were evaluated after removal of the inhibitor. A reduced duration of BL I incubation (22 h) at 20 mu M increased the percentage of oocytes remaining at the GV stage compared to the control group (85% versus 9%, P < 0.05). Blastocyst rates were lower in treatment groups than in the control (44 h) group (0-14% versus 24%; P < 0.05). However, all developing blastocysts possessed similar cell numbers, regardless of the drug-treated or non-treated controls. Taken together, treatment with 20-80 mu M of BL I effectively prevented the resumption of meiosis and polymerization of periooplasmic microtubules. Furthermore, reversibility of the oocytes after reduced duration of BL I treatment was satisfactory. (c) 2006 Elsevier Inc. All rights reserved.
|Appears in Collections:||動物科學系|
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