Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44891
DC FieldValueLanguage
dc.contributor.authorLiu, J.R.en_US
dc.contributor.author余 碧zh_TW
dc.contributor.authorYu, B.en_US
dc.contributor.authorZhao, X.en_US
dc.contributor.authorCheng, K.J.en_US
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:14:03Z-
dc.date.available2014-06-06T08:14:03Z-
dc.identifier.issn0175-7598zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44891-
dc.description.abstractThe aim of this study was to clone and coexpress two rumen fibrolytic enzyme genes in Lactobacillus reuteri. The ability of the genetically modified strain to degrade beta-glucan and xylan was evaluated. The Fibrobacter succinogenes beta-glucanase (1,3-1,4-beta-D-glucan 4-glucanohydrolase [EC 3.2.1.73]) gene and the Neocallimastix patriciarum xylanase gene, xynCDBFV, were constructed to coexpress and secrete under control of the Lactococcus lactis lacA promoter and its secretion signal and then transformed into L. reuteri Pg4, a strain isolated from the gastrointestinal tract of broiler chickens. The transformed L. reuteri strain acquired the capacity to break down soluble beta-glucan and xylan. The introduction of the recombinant plasmids and production of beta-glucanase and xylanase did not affect cell growth. To the best of our knowledge, this is the first report of coexpression of rumen microbial fibrolytic enzyme genes in L. reuteri.en_US
dc.language.isoen_USzh_TW
dc.relationApplied Microbiology and Biotechnologyen_US
dc.relation.ispartofseriesApplied Microbiology and Biotechnology, Volume 77, Issue 1, Page(s) 117-124.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s00253-007-1123-5en_US
dc.subjectrumen microbeen_US
dc.subjectbeta-glucanaseen_US
dc.subjectxylanaseen_US
dc.subjectLactobacillus reuterien_US
dc.subjectthermocellum endoglucanase geneen_US
dc.subjectbacillus-subtilisen_US
dc.subjectcellulase activityen_US
dc.subjectescherichia-colien_US
dc.subjectalpha-amylaseen_US
dc.subjectplasmid dnaen_US
dc.subjectexpressionen_US
dc.subjectplantarumen_US
dc.subjectprobioticsen_US
dc.subjectstabilityen_US
dc.titleCoexpression of rumen microbial beta-glucanase and xylanase genes in Lactobacillus reuterien_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s00253-007-1123-5zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.fulltextno fulltext-
Appears in Collections:動物科學系
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