Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44902
DC FieldValueLanguage
dc.contributor.authorChen, C.H.en_US
dc.contributor.author朱志成zh_TW
dc.contributor.authorStone, L.en_US
dc.contributor.authorJu, J.C.en_US
dc.contributor.authorLien, W.T.en_US
dc.contributor.authorLiu, M.S.en_US
dc.contributor.authorTu, C.F.en_US
dc.contributor.authorLee, K.H.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-06T08:14:04Z-
dc.date.available2014-06-06T08:14:04Z-
dc.identifier.issn0936-6768zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44902-
dc.description.abstractMost studies of mouse cloning successfully achieved activation of the reconstructed oocytes by strontium (Sr) combined with cytochalasin B (CB) treatment. A protein kinase inhibitor, 6-dimethylaminopurine (6-DMAP), was used to inhibit the activity of maturation promoting factor for activation of oocytes, but it has never been successfully applied in mouse cloning. This study investigates the activation efficiency of 6-DMAP in mouse somatic cell nuclear transfer (SCNT). Higher parthenogenetic blastocyst rates (71-72%, p < 0.05) were achieved in the oocytes treated with Sr6D (10 mM Sr combined with 2 mM 6-DMAP for 4 h) and Sr6D + SrCB (Sr6D for 2 h then Sr combined with 5 mu g/ml CB for another 2 h), and a higher rate of hatching and hatched blastocyst was observed in the Sr6D + SrCB group (31%, p < 0.01) compared with other treatment groups (1-8%). For mouse cloning, cumulus cells of enhanced green fluorescent protein (EGFP)-expressed ESC chimera F1 were used as donor nuclei. Following activation, better development of the cloned embryos was observed in Sr6D + SrCB treatment. Moreover, different media, i.e. KSOM-AA, MEM-alpha and MK, for culturing cloned embryos were also compared in this study. Better morula/blastocyst (40%) and blastocyst (29%) rates were achieved in the embryos cultured in MEM-alpha medium (p < 0.05). Consequently, four EGFP cloned mice were generated in the activation treatment containing 6-DMAP following embryo transfer. In conclusion, treatment with 6-DMAP in combination with other activation stimuli successfully activates mouse reconstructed oocytes and support full-term development of the transgenic SCNT cloned embryos.en_US
dc.language.isoen_USzh_TW
dc.relationReproduction in Domestic Animalsen_US
dc.relation.ispartofseriesReproduction in Domestic Animals, Volume 43, Issue 5, Page(s) 547-555.en_US
dc.relation.urihttp://dx.doi.org/10.1111/j.1439-0531.2007.00951.xen_US
dc.subjectparthenogenetic mouse embryosen_US
dc.subjectrepetitive calcium transientsen_US
dc.subjectsomatic-cell nucleien_US
dc.subjectbovine oocytesen_US
dc.subjectmap kinaseen_US
dc.subjectstem-cellsen_US
dc.subjectcloningen_US
dc.subjectcultureen_US
dc.subjectinhibitionen_US
dc.subjecteggen_US
dc.titleTransgenic cloned mice expressing enhanced green fluorescent protein generated by activation stimuli combined with 6-dimethylaminopurineen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1111/j.1439-0531.2007.00951.xzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:動物科學系
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