Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/44931
DC FieldValueLanguage
dc.contributor.authorJu, J.C.en_US
dc.contributor.author朱志成zh_TW
dc.contributor.authorTsay, C.en_US
dc.contributor.authorRuan, C.W.en_US
dc.date2003zh_TW
dc.date.accessioned2014-06-06T08:14:08Z-
dc.date.available2014-06-06T08:14:08Z-
dc.identifier.issn1040-452Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/44931-
dc.description.abstractGerminal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 44 hr in NCSU#23 medium containing different levels of roscovitine (0, 10, 20, 30, 40, 50 muM in Experiment 1 and 0, 40, 60, 80, 100,120 muM in Experiment 2). The COCs were cultured for another 44 hr after removal of the chemical. Twenty oocytes in each group were fixed at 44 hr for immunocytochemical labeling of the cytoskeleton and the rest (similar to20/ group) were fixed at the end of 88 hr after culture. Results showed that the inhibition of the oocyte in the GV stage was not effective when 10-50 muM (Experiment 1) of roscovitine were used (19-34%). When oocytes were released from the inhibitor, similar proportions (70-83%) of oocytes were observed in the MII or advanced stages among treatments. However, when higher concentrations of roscovitine were used (Experiment 2), significantly greater inhibitory effect was observed at the levels of 80-120 muM with 83-91% oocytes being blocked in the GV stage when compared to the control (9%) and the 40-60 W (27-43%) groups (P < 0.05). Although 15-21% of the oocyte showed abnormal MII morphology with aberrant meiotic spindles and/or formation of cytoplasmic microtubules, a substantial number of oocytes resumed meiosis and reached MII stage at 44 hr after removal of this chemical. In Experiment 3, different concentrations of roscovitine (0, 20, 40, and 80 muM) were tested to examine the length of intervals (0, 11, 22, 33, and 44 hr) for an effective inhibition. Results showed that the inhibitory effect was significantly more prominent at 22 hr than that at 33 and 44 hr after roscovitine treatment in all treatment groups (P < 0.05). This study demonstrated that roscovitine-treated oocytes resumed meiosis after removal of the inhibitor. This could provide flexibility for studying porcine oocyte development and embryo cloning and may have application in other species. (C) 2003 Wiley-Liss, Inc.en_US
dc.language.isoen_USzh_TW
dc.relationMolecular Reproduction and Developmenten_US
dc.relation.ispartofseriesMolecular Reproduction and Development, Volume 64, Issue 4, Page(s) 482-491.en_US
dc.relation.urihttp://dx.doi.org/10.1002/mrd.10234en_US
dc.subjectcdc2 kinase inhibitoren_US
dc.subjectIVMen_US
dc.subjectchromatinen_US
dc.subjectmicrotubuleen_US
dc.subjectmicrofilamenten_US
dc.subjectcyclin-dependent kinasesen_US
dc.subjectin-vitro maturationen_US
dc.subjectgerminal vesicleen_US
dc.subjectbreakdownen_US
dc.subjectmatured bovine oocytesen_US
dc.subjectporcine oocytesen_US
dc.subjectparthenogeneticen_US
dc.subjectdevelopmenten_US
dc.subjectchemical inhibitorsen_US
dc.subjectmeiotic maturationen_US
dc.subjectmammalian oocytesen_US
dc.subjectmicrotubule organizationen_US
dc.titleAlterations and reversibility in the chromatin, cytoskeleton and development of pig oocytes treated with roscovitineen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1002/mrd.10234zh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
Appears in Collections:動物科學系
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