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|標題:||Nuclear and cytoskeletal dynamics during oocyte maturation and development of somatic cell cloned pig embryos injected with membrane disintegrated donor cells||作者:||Lin, T.A.
|關鍵字:||microtubules;microfilaments;pig oocytes;somatic cell nuclear transfer;in-vitro maturation;activated protein-kinase;intracytoplasmic;microinjection;cumulus cells;fertilization;microtubule;microfilaments;actin;fetal;organization||Project:||Animal Reproduction Science||期刊/報告no：:||Animal Reproduction Science, Volume 103, Issue 1-2, Page(s) 107-119.||摘要:||
The objectives of this study were to characterize the nuclear and cytoskeletal changes of pig oocytes during in vitro maturation (IVM) and the development of the reconstructed embryos after injection with membrane intact or disintegrated donor cells. Cumulus-oocyte complexes (COCs) were collected from abattoir ovaries by follicle (2-8 mm) aspiration. In Experiment 1, COCs were cultured in NCSU-23 medium for 0, 11, 22, 33, and 44h. Oocytes were fixed at different time points for nuclear and cytoskeletal labeling. Forty-three percent and 75% oocytes progressed to MII stage at 33 and 44h after IVM culture, respectively. Dynamic shift of spindle and cytoplasmic microtububes was evident. In Experiment 2, matured oocytes were injected with either the whole cumulus cell with or without intact cell membranes after enucleation. The reconstructed oocytes were fixed at 0, 2, or 4 h after cell injection for nuclear and cytoskeletal evaluation. When an intact cumulus cell was injected, the injected cell remained intact within 4 h after injection. When a cell with disintegrated membrane was injected, 59-63% (n = 146) of the injected cell underwent premature chromosome condensation (PCC). In Experiment 3, the reconstructed pig oocytes received membrane-disintegrated cumulus cells or fetal fibroblasts were Cultured in PZM medium. The blastocyst rate of the fibroblast-injected embryos was 10%, which was lower than the non-cloned parthenotes (33%, P < 0.05) but higher than the cumulus cell-injected embryos (2.7%). These results suggest that pig oocytes are subjected to nuclear and cytoskeletal reorganization during maturation. Pig oocytes injected with membrane-disintegrated fibroblast cells support better blastocyst development of the cloned embryos. (C) 2006 Elsevier B.V. All rights reserved.
|Appears in Collections:||動物科學系|
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