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標題: Use of specific primers based on the 16S-23S internal transcribed spacer (ITS) region for the screening Bifidobacterium adolescentis in yogurt products and human stool samples
作者: Tsai, C.C.
余 碧
Lai, C.H.
Yu, B.
Tsen, H.Y.
關鍵字: Bifidobacterium adolescentis;polymerase chain reaction;16S-23S;internal transcribed spacers;16s ribosomal-rna;pcr primers;colony hybridization;identification;gene;food;infants;origins
Project: Anaerobe
期刊/報告no:: Anaerobe, Volume 14, Issue 4, Page(s) 219-223.
Effective methods for the identification and enumeration of lactic acid producing bacteria (LAB) cells are important for the quality control and assurance of probiotic products. In this study, we designed a polymerase chain reaction (PCR) primer set from the sequence in 16S-23S internal transcribed spacer (ITS) region and used it for the specific detection of Bifidobacterium adolescentis, one of the Bifidobacterium species used in probiotics. Specificity of the PCR primers, i.e., bits-1/bits-2, was assured by assay strains of B. adolescentis, other Bifidobacterium species, and strains of non-Bifidobacterium spp. Coupled with the use of a known primer set specific for Bifidobacterium species, Bifidobacterium strains and B. adolescentis could be identified from LAB strains in fermented dairy products and human fecal samples. (C) 2008 Elsevier Ltd. All rights reserved.
ISSN: 1075-9964
DOI: 10.1016/j.anaerobe.2008.05.001
Appears in Collections:動物科學系

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