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標題: Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous beta-glucanase as a probiotic in poultry diets based on barley
作者: Yu, B.
余 碧
Liu, J.R.
Hsiao, F.S.
Chiou, R.S.
關鍵字: beta-glucanase;Lactobacillus reuteri Pg4;probiotic;broiler;performance;growth-performance;intestinal microflora;broiler-chickens;digestibilities;gut
Project: Animal Feed Science and Technology
期刊/報告no:: Animal Feed Science and Technology, Volume 141, Issue 1-2, Page(s) 82-91.
The aim of this study was to evaluate the effectiveness of Lactobacillus reuteri Pg4 transformants (TLB) harbouring the heterologous P-glucanase gene as a probiotic supplement in a barley-based poultry diet. Four-hundred broiler chicks were allocated to two treatment groups with or without supplementation with 10(8) CFU/g TLB to investigate growth performance, digesta. viscosity and intestinal microflora. Supplementation of the barley-based diet with TLB decreased digesta viscosity and improved body weight gain (P<0.05) of broilers from 0 to 37 days of age. Furthermore, a higher total anaerobic and Lactobacillus count was observed at 37 days of age in all segments of gastrointestinal tract of chicks fed TLB as compared to control chicks (P<0.05). Radial enzyme diffusion methodology was used to demonstrate that the Lactobacillus spp. randomly isolated from the GIT of broilers fed TLB possessed P-glucanase secretion capability. The results indicate that the transformed strain survives transit through the stomach and intestine. In conclusion, the transformed Lactobacillus reuteri Pg4 can survive and secrete P-glucanase in the broiler GIT, and decrease digesta viscosity and enhance weight gain in birds fed a barley-based diet. Therefore, the transformed Lactobacillus reuteri Pg4 harbouring heterologous P-glucanase gene has potential as a poultry probiotic. (C) 2007 Elsevier B.V. All rights reserved.
ISSN: 0377-8401
DOI: 10.1016/j.anifeedsci.2007.04.010
Appears in Collections:動物科學系

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