Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/45025
DC FieldValueLanguage
dc.contributor.authorLiu, J.R.en_US
dc.contributor.author余 碧zh_TW
dc.contributor.authorYu, B.en_US
dc.contributor.authorLin, S.H.en_US
dc.contributor.authorCheng, K.J.en_US
dc.contributor.authorChen, Y.C.en_US
dc.date2005zh_TW
dc.date.accessioned2014-06-06T08:14:17Z-
dc.date.available2014-06-06T08:14:17Z-
dc.identifier.issn0378-1097zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/45025-
dc.description.abstractA relatively newly defined xylanase gene, xynR8, was obtained directly from a mixed DNA sample prepared from unpurified rumen fungal cultures by PCR amplification. The DNA sequence of xynR8 revealed that the gene was 884 bp in size and encoded amino acid sequences with a molecular weight of 27.9 kDa. XynR8 belonged to glycosyl hydrolase family 11, and the catalytic site residues were also found in its amino acid sequence. The main hydrolysis products of XynR8 were xylobiose, xylotriose and xylotetrose, which indicated that it belonged to the endoxylanases. The xynR8 gene was constructed so as to express and secrete under the control of the Lactococcus lactis lac A promoter and its secretion signal, and was transformed into L. reuteri Pg4, a strain isolated from the gastrointestinal tract of broiler chickens. The L. reuteri transformants harboring xynR8 not only acquired the capacity to break down xylan, but also maintained their high adhesion efficiency to mucin and mucus and their resistance to bile salts and acid. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationFems Microbiology Lettersen_US
dc.relation.ispartofseriesFems Microbiology Letters, Volume 251, Issue 2, Page(s) 233-241.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.femsle.2005.08.008en_US
dc.subjectLactobacillus reuterien_US
dc.subjectOrpinomycesen_US
dc.subjectrumen fungien_US
dc.subjectxylanase geneen_US
dc.subjectprobiotic lactobacillien_US
dc.subjectbroiler-chickensen_US
dc.subjectplantarumen_US
dc.subjectcellulasesen_US
dc.subjectadhesionen_US
dc.subjectpoultryen_US
dc.subjectbindingen_US
dc.subjectdomainen_US
dc.subjectlactisen_US
dc.subjectwheaten_US
dc.titleDirect cloning of a xylanase gene from the mixed genomic DNA of rumen fungi and its expression in intestinal Lactobacillus reuterien_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.femsle.2005.08.008zh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
Appears in Collections:動物科學系
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