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標題: Expression of Lactobacillus reuteri Pg4 Collagen-Binding Protein Gene in Lactobacillus casei ATCC 393 Increases Its Adhesion Ability to Caco-2 Cells
作者: Hsueh, H.Y.
余 碧
Yueh, P.Y.
Yu, B.
Zhao, X.
Liu, J.R.
關鍵字: Collagen-binding protein;adhesion;Lactobacillus reuteri;Lactobacillus;casei;diarrheagenic escherichia-coli;epithelial-cells;fermentum br11;gastrointestinal-tract;chicken intestine;surface-proteins;probiotics;adherence;strains;genome
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 58, Issue 23, Page(s) 12182-12191.
The collagen-binding protein gene cnb was cloned from the probiotic Lactobacillus reuteri strain Pg4. The DNA sequence of the cnb gene (792 bp) has an open reading frame encoding 263 amino acids with a calculated molecular weight of 28.5 kDa. The cnb gene was constructed so as to constitutively express under the control of the Lactococcus lactis lacA promoter and was transformed into Lactobacillus casei ATCC 393, a strain isolated from dairy products with poor ability to adhere to intestinal epithelial cells. Confocal immunofluorescence microscopic and flow cytometric analysis of the transformed strain Lb. casei pNZ-cnb indicated that Cnb was displayed on its cell surface. Lb. casei pNZ-cnb not only showed a higher ability to adhere to Caco-2 cells but also exhibited a higher competition ability against Escherichia coli O157:H7 and Listeria monocytogenes adhesion to Caco-2 cells than Lb. casei ATCC 393.
ISSN: 0021-8561
DOI: 10.1021/jf1035756
Appears in Collections:動物科學系

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